AF5647

antibody from R&D Systems

Targeting: FOXP2 CAGH44, SPCH1, TNRC10

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF5647

Provider

R&D Systems

Product name

Human/Mouse FoxP2 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human and mouse FoxP2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) FoxP1, rhFoxP3, and rhFoxP4 is observed.

Reactivity

Human, Mouse

Host

Sheep

Conjugate

Unconjugated

Antigen sequence

O15409


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Chemosensory modulation of neural circuits for sodium appetite.

Lee S, Augustine V, Zhao Y, Ebisu H, Ho B, Kong D, Oka Y
Nature 2019 Apr;568(7750):93-97

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Efferent projections of excitatory and inhibitory preBötzinger Complex neurons.

Yang CF, Feldman JL
The Journal of comparative neurology 2018 Jun 1;526(8):1389-1402

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Kölliker-Fuse GABAergic and glutamatergic neurons project to distinct targets.

Geerling JC, Yokota S, Rukhadze I, Roe D, Chamberlin NL
The Journal of comparative neurology 2017 Jun 1;525(8):1844-1860

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Barrington's nucleus: Neuroanatomic landscape of the mouse "pontine micturition center".

Verstegen AMJ, Vanderhorst V, Gray PA, Zeidel ML, Geerling JC
The Journal of comparative neurology 2017 Jul 1;525(10):2287-2309

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HSD2 neurons in the hindbrain drive sodium appetite.

Jarvie BC, Palmiter RD
Nature neuroscience 2017 Feb;20(2):167-169

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human FoxP2 by Western Blot. Western blot shows lysates of A172 human glioblastoma cell line and human lung tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse FoxP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5647) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FoxP2 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunocytochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

FoxP2 in HeLa Human Cell Line. FoxP2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using 10 µg/mL Sheep Anti-Human/Mouse FoxP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5647) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.