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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
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- Product number
- H00002617-D01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00002617-D01, RRID:AB_10735340
- Product name
- GARS MaxPab rabbit polyclonal antibody (D01)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against a full-length human GARS protein.
- Antigen sequence
MDGAGAEEVLAPLRLAVRQQGDLVRKLKEDKAPQV
DVDKAVAELKARKRVLEAKELALQPKDDIVDRAKM
EDTLKRRFFYDQAFAIYGGVSGLYDFGPVGCALKN
NIIQTWRQHFIQEEQILEIDCTMLTPEPVLKTSGH
VDKFADFMVKDVKNGECFRADHLLKAHLQKLMSDK
KCSVEKKSEMESVLAQLDNYGQQELADLFVNYNVK
SPITGNDLSPPVSFNLMFKTFIGPGGNMPGYLRPE
TAQGIFLNFKRLLEFNQGKLPFAAAQIGNSFRNEI
SPRSGLIRVREFTMAEIEHFVDPSEKDHPKFQNVA
DLHLYLYSAKAQVSGQSARKMRLGDAVEQGVINNT
VLGYFIGRIYLYLTKVGISPDKLRFRQHMENEMAH
YACDCWDAESKTSYGWIEIVGCADRSCYDLSCHAR
ATKVPLVAEKPLKEPKTVNVVQFEPSKGAIGKAYK
KDAKLVMEYLAICDECYITEMEMLLNEKGEFTIET
EGKTFQLTKDMINVKRFQKTLYVEEVVPNVIEPSF
GLGRIMYTVFEHTFHVREGDEQRTFFSFPAVVAPF
KCSVLPLSQNQEFMPFVKELSEALTRHGVSHKVDD
SSGSIGRRYARTDEIGVAFGVTIDFDTVNKTPHTA
TLRDRDSMRQIRAEISELPSIVQDLANGNITWADV
EARYPLFEGQETGKKETIEE- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of GARS expression in transfected 293T cell line (H00002617-T01) by GARS MaxPab polyclonal antibody.Lane 1: GARS transfected lysate(77.5 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of GARS transfected lysate using anti-GARS MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with GARS purified MaxPab mouse polyclonal antibody (B01P) (H00002617-B01P).
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
