10476-1-AP

antibody from Invitrogen Antibodies

Targeting: BRD8 p120, SMAP

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

10476-1-AP

Provider

Invitrogen Antibodies

Product name

BRD8 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: TVKQERLDF EETENKGIHE LVDIREPSAE IKVEPAEPEP VISGAEIVAG VVPATSMEPP ELRSQDLDEE LGSTAAGEIV EADVAIGKGD ETPLTNVKTE ASPESMLSPS HGSNPIEDPL EAETQHKFEM SDSLKEESGT IFGSQIKDAP GEDEEEDGVS EAASLEEPKE EDQGEGYLSE MDNEPPVSES DDGFSIHNAT LQSHTLADSI PSSPASSQFS VCSEDQEAIQ AQKIWKKAIM LVWRAAANHR YANVFLQPVT DDIAPGYHSI VQRPMDLSTI KKNIENGLIR STAEFQRDIM LMFQNAVMYN SSDHDVYHMA VEMQRDVLEQ IQQFLATQLI MQTSESGISA KSLRGRDSTR KQDASEKDSV PMGSPAFLLS LFDGGTRGRR CAIEADMKMK K (521-920 aa encoded by BC008039)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.32 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human brain tissue were subjected to SDS PAGE followed by western blot with 10476-1-AP (BRD8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human brain tissue were subjected to SDS PAGE followed by western blot with 10476-1-AP (BRD8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 10476-1-AP (BRD8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 10476-1-AP (BRD8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-BRD8 (IP:10476-1-AP, 4ug; Detection:10476-1-AP 1:300) with HeLa cells lysate 1200ug.