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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- 60121-1-IG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BRD8 Monoclonal Antibody (5B6D6)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Immunogen sequence: TVKQERLDF EETENKGIHE LVDIREPSAE IKVEPAEPEP VISGAEIVAG VVPATSMEPP ELRSQDLDEE LGSTAAGEIV EADVAIGKGD ETPLTNVKTE ASPESMLSPS HGSNPIEDPL EAETQHKFEM SDSLKEESGT IFGSQIKDAP GEDEEEDGVS EAASLEEPKE EDQGEGYLSE MDNEPPVSES DDGFSIHNAT LQSHTLADSI PSSPASSQFS VCSEDQEAIQ AQKIWKKAIM LVWRAAANHR YANVFLQPVT DDIAPGYHSI VQRPMDLSTI KKNIENGLIR STAEFQRDIM LMFQNAVMYN SSDHDVYHMA VEMQRDVLEQ IQQFLATQLI MQTSESGISA KSLRGRDSTR KQDASEKDSV PMGSPAFLLS LFDGGTRGRR CAIEADMKMK K (521-920 aa encoded by BC008039)
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 5B6D6
- Vial size
- 150 µL
- Concentration
- 1.47 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- WB result of BRD8 antibody (60121-1-IG, 1:8000) with si-Control and si-BRD8 transfected HeLa cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60121-1-IG ( BRD8 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( 4% PFA ) fixed HEK-293 cells using 60121-1-IG (BRD8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 60121-1-Ig (BRD8 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 60121-1-IG ( BRD8 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
