AP19180c

antibody from Abgent

Targeting: RHOXF2B

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP19180c

Provider

Abgent

Product name

RHOXF2B Antibody (Center)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

0.4 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

RHOXF2B Antibody (Center) (Cat. #AP19180c) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the RHOXF2B antibody detected the RHOXF2B protein (arrow).

Primary Ab dilution

1:1000

Immunocytochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Fluorescent confocal image of A549 cell stained with RHOXF2B Antibody (Center)(Cat#AP19180c).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RHOXF2B primary antibody (1:25, 1 h at 37!). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37!).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37!). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).RHOXF2B immunoreactivity is localized to Nucleus significantly."

Primary Ab dilution

1:10~50