NB500-514

antibody from Novus Biologicals

Targeting: STAT1 ISGF-3, STAT91

Western blot (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

NB500-514

Provider

Novus Biologicals

Proper citation

Novus Cat#NB500-514, RRID:AB_10002087

Product name

Mouse Monoclonal STAT1 Antibody

Antibody type

Monoclonal

Description

Protein A purified. The antibody SM1 recognizes an epitope included within amino acids 721-733 of STAT1, a 91 kDa transcriptional factor involved in a variety of systems including antiviral responses and interferon alpha (IFN-alpha) and gamma (IFN-gamma) signal transduction.

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Concentration

1.0 mg/ml

Storage

Store at 4C. Do not freeze.

References

Submitted references

Interferon inducibility of STAT 1 activation and its prognostic significance in melanoma patients.

Boudný V, Kocák I, Lauerová L, Kovarík J
Folia biologica 2003;49(4):142-6

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Malignant melanoma associates with deficient IFN-induced STAT 1 phosphorylation.

Kovarik J, Boudny V, Kocak I, Lauerova L, Fait V, Vagundova M
International journal of molecular medicine 2003 Sep;12(3):335-40

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: STAT1 Antibody (SM1) [NB500-514] - Fig. 1: Induction of phosphorylation of STAT1 at Ser727 in human malignant melanoma cells (short-term culture derived from a patient) in response to interferons. Subconfluent cells were serum-starved before exposure to activation dosages of IFN-gamma (10 ng/ml) and IFN-alpha (1000 IU/ml and 5000 IU/ml). Western blotting analysis of cell extracts shows detection of phosphorylated STAT1 (Ser727) by the antibody PSM1 (upper panel) and total STAT1 level by the antibody SM1 (lower panel).