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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Validations
- Western blot [2]
- Flow cytometry [1]
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- Product number
- AF2894 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse Phospho-STAT1 (Y701) Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human STAT1 when phosphorylated at Y701 in Western blots.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 50 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references PD-L1 blockade enhances response of pancreatic ductal adenocarcinoma to radiotherapy.
Molecular determinants of plaque size as an indicator of dengue virus attenuation.
Thymic DCs derived IL-27 regulates the final maturation of CD4(+) SP thymocytes.
Annexin V incorporated into influenza virus particles inhibits gamma interferon signaling and promotes viral replication.
The RAS/Raf1/MEK/ERK signaling pathway facilitates VSV-mediated oncolysis: implication for the defective interferon response in cancer cells.
Azad A, Yin Lim S, D'Costa Z, Jones K, Diana A, Sansom OJ, Kruger P, Liu S, McKenna WG, Dushek O, Muschel RJ, Fokas E
EMBO molecular medicine 2017 Feb;9(2):167-180
EMBO molecular medicine 2017 Feb;9(2):167-180
Molecular determinants of plaque size as an indicator of dengue virus attenuation.
Goh KC, Tang CK, Norton DC, Gan ES, Tan HC, Sun B, Syenina A, Yousuf A, Ong XM, Kamaraj US, Cheung YB, Gubler DJ, Davidson A, St John AL, Sessions OM, Ooi EE
Scientific reports 2016 May 17;6:26100
Scientific reports 2016 May 17;6:26100
Thymic DCs derived IL-27 regulates the final maturation of CD4(+) SP thymocytes.
Tang H, Zhang J, Sun X, Qian X, Zhang Y, Jin R
Scientific reports 2016 Jul 29;6:30448
Scientific reports 2016 Jul 29;6:30448
Annexin V incorporated into influenza virus particles inhibits gamma interferon signaling and promotes viral replication.
Berri F, Haffar G, Lê VB, Sadewasser A, Paki K, Lina B, Wolff T, Riteau B
Journal of virology 2014 Oct;88(19):11215-28
Journal of virology 2014 Oct;88(19):11215-28
The RAS/Raf1/MEK/ERK signaling pathway facilitates VSV-mediated oncolysis: implication for the defective interferon response in cancer cells.
Noser JA, Mael AA, Sakuma R, Ohmine S, Marcato P, Lee PW, Ikeda Y
Molecular therapy : the journal of the American Society of Gene Therapy 2007 Aug;15(8):1531-6
Molecular therapy : the journal of the American Society of Gene Therapy 2007 Aug;15(8):1531-6
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Phospho-STAT1 (Y701) by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line untreated (-) or treated (+) with Recombinant Human IFN-alpha 2a for 20 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse Phospho-STAT1 (Y701) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2894), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-STAT1 (Y701) at approximately 94 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Phospho-STAT1 (Y701) by Simple WesternTM. Simple Western lane view shows lysates of Daudi human Burkitt's lymphoma cell line untreated (-) or treated (+) with Recombinant Human IFN-alpha A (Catalog # 11100-1) for 20 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-STAT1 (Y701) at approximately 86 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human/Mouse Phospho-STAT1 (Y701) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2894). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Phospho-STAT1 in IFN-alpha-treated Daudi Human Cell Line by Flow Cytometry. Daudi human Burkitt's lymphoma cell line was unstimulated (light orange filled histogram) or treated with 500 units/mL Recombinant Human IFN-alpha (Catalog # 11100-1) for 20 minutes (dark orange filled histogram), then stained with Rabbit Anti-Human/Mouse Phospho-STAT1 (Y701) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2894) or control antibody (Catalog # AB-105-C, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol.
