Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [3]
- Other assay [1]
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Validation data
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- Product number
- PA5-42001 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- C4BPA Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide sequence: QVLTGKRLMQ CLPNPEDVKM ALEVYKLSLE IEQLELQRDS ARQSTLDKEL
- Concentration
- 0.5 mg/mL
Submitted references Bacillus anthracis Poly-γ-D-Glutamate Capsule Inhibits Opsonic Phagocytosis by Impeding Complement Activation.
Ail provides multiple mechanisms of serum resistance to Yersinia pestis.
Sharma S, Bhatnagar R, Gaur D
Frontiers in immunology 2020;11:462
Frontiers in immunology 2020;11:462
Ail provides multiple mechanisms of serum resistance to Yersinia pestis.
Thomson JJ, Plecha SC, Krukonis ES
Molecular microbiology 2019 Jan;111(1):82-95
Molecular microbiology 2019 Jan;111(1):82-95
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HepG2 whole cell lysate using an anti-C4BP polyclonal antibody (Product # PA5-42001). Lane 1) Primary Antibody; Lane 2) Primary Antibody + Blocking Peptide.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human MCF-7 cell lysate using an anti-C4BP polyclonal antibody (Product # PA5-42001).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-C4BPA Polyclonal Antibody (Product # PA5-42001) and bands corresponding to 93kDa and 52kDa of C4BPA were observed in the reported high expressing models- Hep G2, Mouse Epididymis, Mouse Liver, Rat Epididymis and Rat Liver in comparison to DU 145, U-937, BeWO and Mouse Lung . Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), DU 145 (Lane 2), U-937 (Lane 3), BeWO (Lane 4), tissue extracts (30ug lysate) of Mouse Lungs (Lane 4), Mouse Epididymis (Lane 5), Mouse Liver (Lane 6), Rat Epididymis (Lane 7) and Rat Liver (Lane 8) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001).The blot was probed with the antibody (0.75 µg/ml) and detected by ECL with Goat anti-Rabbit IgG (H+L), Superclonal™ secondary, HRP (Product # A27036, 1:4000 dilution). ECL was performed using Novex® ECL Kit (Product # WP20005). C4 protein, composed of alpha, beta and gamma chain, forms a heterotrimer of ~93 kDa alpha chains via disulphide linkage as detected by this antibody and a ~52 kDa protein corresponding to mouse and rat specific isoforms of C4BPA. It is also reported to be secreted by the epithelial cells surrounding the epididymis for sperm maturation in a region-specific, androgen dependent and species specific manner in addition to being synthesized in the liver.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL