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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- TA501348 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA501348, RRID:AB_11129997
- Product name
- C21orf59 mouse monoclonal antibody, clone OTI2E5 (formerly 2E5)
- Antibody type
- Monoclonal
- Description
- C21orf59 mouse monoclonal antibody, clone OTI2E5 (formerly 2E5)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- CFAP298
- Isotype
- IgG
- Antibody clone number
- OTI2E5
- Vial size
- 100 µl
- Concentration
- 0.75 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY C21orf59 (RC200169, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-C21orf59.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-C21orf59 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-C21orf59 mouse monoclonal antibody (TA501348) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY C21orf59(RC200169).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Jurkat cells, using anti-C21orf59 antibody(TA501348),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
- Validation comment
- FC
