Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Flow cytometry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- TA501350 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA501350, RRID:AB_11126261
- Product name
- C21orf59 mouse monoclonal antibody, clone OTI2H5 (formerly 2H5)
- Antibody type
- Monoclonal
- Description
- C21orf59 mouse monoclonal antibody, clone OTI2H5 (formerly 2H5)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- CFAP298
- Isotype
- IgG
- Antibody clone number
- OTI2H5
- Vial size
- 100 µl
- Concentration
- 0.55 mg/ml
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY C21orf59 (RC200169, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-C21orf59.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 6 different cell lines by using anti-C21orf59 monoclonal antibody.(1:200)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-C21orf59 mouse monoclonal antibody (TA501350) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY C21orf59(RC200169).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Jurkat cells, using anti-C21orf59 antibody(TA501350),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC200169 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-C21orf59 antibody(TA501350), and then analyzed by flow cytometry.
- Validation comment
- FC
