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Western blot
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ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [16]
- Comments [0]
- Validations
- Immunocytochemistry [4]
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- Product number
- ADI-AAM-118-E - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#ADI-AAM-118-E, RRID:AB_2038943
- Product name
- Caspase-8 monoclonal antibody (5F7)
- Antibody type
- Monoclonal
- Antigen
- Recombinant protein fragment
- Description
- Protein A-affinity purified.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 5F7
- Vial size
- 100 μg
- Storage
- -20°C
Submitted references dsRNA induces apoptosis through an atypical death complex associating TLR3 to caspase-8.
Silibinin sensitizes human glioma cells to TRAIL-mediated apoptosis via DR5 up-regulation and down-regulation of c-FLIP and survivin.
p53-R273H gains new function in induction of drug resistance through down-regulation of procaspase-3.
Death-receptor activation halts clathrin-dependent endocytosis.
Synthetic triterpenoids cooperate with tumor necrosis factor-related apoptosis-inducing ligand to induce apoptosis of breast cancer cells.
Molecular determinants of kinase pathway activation by Apo2 ligand/tumor necrosis factor-related apoptosis-inducing ligand.
Interchain proteolysis, in the absence of a dimerization stimulus, can initiate apoptosis-associated caspase-8 activation.
Role of murine cytomegalovirus US22 gene family members in replication in macrophages.
Cephalostatin 1 selectively triggers the release of Smac/DIABLO and subsequent apoptosis that is characterized by an increased density of the mitochondrial matrix.
Caspase-dependent cleavage of c-Abl contributes to apoptosis.
Multiple pathways of TWEAK-induced cell death.
A cytomegalovirus-encoded inhibitor of apoptosis that suppresses caspase-8 activation.
Matrix attachment regulates Fas-induced apoptosis in endothelial cells: a role for c-flip and implications for anoikis.
A cytomegalovirus-encoded mitochondria-localized inhibitor of apoptosis structurally unrelated to Bcl-2.
ATP-dependent steps in apoptotic signal transduction.
Inhibition of Fas-induced apoptosis by Bcl-2.
Estornes Y, Toscano F, Virard F, Jacquemin G, Pierrot A, Vanbervliet B, Bonnin M, Lalaoui N, Mercier-Gouy P, Pachéco Y, Salaun B, Renno T, Micheau O, Lebecque S
Cell death and differentiation 2012 Sep;19(9):1482-94
Cell death and differentiation 2012 Sep;19(9):1482-94
Silibinin sensitizes human glioma cells to TRAIL-mediated apoptosis via DR5 up-regulation and down-regulation of c-FLIP and survivin.
Son YG, Kim EH, Kim JY, Kim SU, Kwon TK, Yoon AR, Yun CO, Choi KS
Cancer research 2007 Sep 1;67(17):8274-84
Cancer research 2007 Sep 1;67(17):8274-84
p53-R273H gains new function in induction of drug resistance through down-regulation of procaspase-3.
Wong RP, Tsang WP, Chau PY, Co NN, Tsang TY, Kwok TT
Molecular cancer therapeutics 2007 Mar;6(3):1054-61
Molecular cancer therapeutics 2007 Mar;6(3):1054-61
Death-receptor activation halts clathrin-dependent endocytosis.
Austin CD, Lawrence DA, Peden AA, Varfolomeev EE, Totpal K, De Mazière AM, Klumperman J, Arnott D, Pham V, Scheller RH, Ashkenazi A
Proceedings of the National Academy of Sciences of the United States of America 2006 Jul 5;103(27):10283-8
Proceedings of the National Academy of Sciences of the United States of America 2006 Jul 5;103(27):10283-8
Synthetic triterpenoids cooperate with tumor necrosis factor-related apoptosis-inducing ligand to induce apoptosis of breast cancer cells.
Hyer ML, Croxton R, Krajewska M, Krajewski S, Kress CL, Lu M, Suh N, Sporn MB, Cryns VL, Zapata JM, Reed JC
Cancer research 2005 Jun 1;65(11):4799-808
Cancer research 2005 Jun 1;65(11):4799-808
Molecular determinants of kinase pathway activation by Apo2 ligand/tumor necrosis factor-related apoptosis-inducing ligand.
Varfolomeev E, Maecker H, Sharp D, Lawrence D, Renz M, Vucic D, Ashkenazi A
The Journal of biological chemistry 2005 Dec 9;280(49):40599-608
The Journal of biological chemistry 2005 Dec 9;280(49):40599-608
Interchain proteolysis, in the absence of a dimerization stimulus, can initiate apoptosis-associated caspase-8 activation.
Murphy BM, Creagh EM, Martin SJ
The Journal of biological chemistry 2004 Aug 27;279(35):36916-22
The Journal of biological chemistry 2004 Aug 27;279(35):36916-22
Role of murine cytomegalovirus US22 gene family members in replication in macrophages.
Ménard C, Wagner M, Ruzsics Z, Holak K, Brune W, Campbell AE, Koszinowski UH
Journal of virology 2003 May;77(10):5557-70
Journal of virology 2003 May;77(10):5557-70
Cephalostatin 1 selectively triggers the release of Smac/DIABLO and subsequent apoptosis that is characterized by an increased density of the mitochondrial matrix.
Dirsch VM, Müller IM, Eichhorst ST, Pettit GR, Kamano Y, Inoue M, Xu JP, Ichihara Y, Wanner G, Vollmar AM
Cancer research 2003 Dec 15;63(24):8869-76
Cancer research 2003 Dec 15;63(24):8869-76
Caspase-dependent cleavage of c-Abl contributes to apoptosis.
Barilà D, Rufini A, Condò I, Ventura N, Dorey K, Superti-Furga G, Testi R
Molecular and cellular biology 2003 Apr;23(8):2790-9
Molecular and cellular biology 2003 Apr;23(8):2790-9
Multiple pathways of TWEAK-induced cell death.
Nakayama M, Ishidoh K, Kayagaki N, Kojima Y, Yamaguchi N, Nakano H, Kominami E, Okumura K, Yagita H
Journal of immunology (Baltimore, Md. : 1950) 2002 Jan 15;168(2):734-43
Journal of immunology (Baltimore, Md. : 1950) 2002 Jan 15;168(2):734-43
A cytomegalovirus-encoded inhibitor of apoptosis that suppresses caspase-8 activation.
Skaletskaya A, Bartle LM, Chittenden T, McCormick AL, Mocarski ES, Goldmacher VS
Proceedings of the National Academy of Sciences of the United States of America 2001 Jul 3;98(14):7829-34
Proceedings of the National Academy of Sciences of the United States of America 2001 Jul 3;98(14):7829-34
Matrix attachment regulates Fas-induced apoptosis in endothelial cells: a role for c-flip and implications for anoikis.
Aoudjit F, Vuori K
The Journal of cell biology 2001 Feb 5;152(3):633-43
The Journal of cell biology 2001 Feb 5;152(3):633-43
A cytomegalovirus-encoded mitochondria-localized inhibitor of apoptosis structurally unrelated to Bcl-2.
Goldmacher VS, Bartle LM, Skaletskaya A, Dionne CA, Kedersha NL, Vater CA, Han JW, Lutz RJ, Watanabe S, Cahir McFarland ED, Kieff ED, Mocarski ES, Chittenden T
Proceedings of the National Academy of Sciences of the United States of America 1999 Oct 26;96(22):12536-41
Proceedings of the National Academy of Sciences of the United States of America 1999 Oct 26;96(22):12536-41
ATP-dependent steps in apoptotic signal transduction.
Eguchi Y, Srinivasan A, Tomaselli KJ, Shimizu S, Tsujimoto Y
Cancer research 1999 May 1;59(9):2174-81
Cancer research 1999 May 1;59(9):2174-81
Inhibition of Fas-induced apoptosis by Bcl-2.
Kawahara A, Kobayashi T, Nagata S
Oncogene 1998 Nov 19;17(20):2549-54
Oncogene 1998 Nov 19;17(20):2549-54
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Supportive validation
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Western Blot analysis of Caspase-8 fragments in apoptosis induced Jurkat cells using Caspase-8, mAb (5F7) (Prod. No. ADI-AAM-118). The Jurkat cells were treated with a FAS monoclonal antibody for the indicated time
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Western blot analysis of Jurkat (1), Raji (2), HeLa (3) and WR19L (4) probed with Caspase-8, mAb (5F7).
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Immunohistochemistry staining of human breast tissue using anti-Caspase-8 antibody (prod.no. ADI-AAM-118 clone 5F7, 1:50) showed positive staining in both cytoplasm and nucleus of breast epithelial and myoepithelial cells. Image taken at magnification of 63x.
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Immunohistochemistry staining of human breast tissue using anti-Caspase-8 antibody (prod.no. ADI-AAM-118 clone 5F7, 1:50) showed positive staining in both cytoplasm and nucleus of breast epithelial and myoepithelial cells. Image taken at magnification of 63x.
