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- Antibody Data
- Antigen structure
- References [21]
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- Validations
- Immunocytochemistry [2]
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- Product number
- ALX-804-447-C100 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#ALX-804-447-C100, RRID:AB_2050952
- Product name
- Caspase-8 (mouse) monoclonal antibody (1G12)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full length protein
- Description
- Protein G-affinity purified.
- Reactivity
- Mouse
- Host
- Rat
- Isotype
- IgG
- Antibody clone number
- 1G12
- Vial size
- 100 μg
- Storage
- -20°C
- Handling
- For long term storage, aliquot and freeze at -20¡C. Avoid freeze/thaw cycles.
Submitted references Punicalagin exerts protective effect against high glucose-induced cellular stress and neural tube defects.
NIK promotes tissue destruction independently of the alternative NF-κB pathway through TNFR1/RIP1-induced apoptosis.
Cellular-FLIP, Raji isoform (c-FLIP R) modulates cell death induction upon T-cell activation and infection.
AIM2/ASC triggers caspase-8-dependent apoptosis in Francisella-infected caspase-1-deficient macrophages.
Protease activity of procaspase-8 is essential for cell survival by inhibiting both apoptotic and nonapoptotic cell death dependent on receptor-interacting protein kinase 1 (RIP1) and RIP3.
Mechanisms of necroptosis in T cells.
Apoptosis and non-inflammatory phagocytosis can be induced by mitochondrial damage without caspases.
The death domain of FADD is essential for embryogenesis, lymphocyte development, and proliferation.
Antigen-mediated T cell expansion regulated by parallel pathways of death.
A cell-type-specific requirement for IFN regulatory factor 5 (IRF5) in Fas-induced apoptosis.
Mutation of a self-processing site in caspase-8 compromises its apoptotic but not its nonapoptotic functions in bacterial artificial chromosome-transgenic mice.
Blockade of tumor necrosis factor-induced Bid cleavage by caspase-resistant Rb.
FLIP(L) protects neurons against in vivo ischemia and in vitro glucose deprivation-induced cell death.
Loss of caspase-9 provides genetic evidence for the type I/II concept of CD95-mediated apoptosis.
Apaf-1 and caspase-9 are required for cytokine withdrawal-induced apoptosis of mast cells but dispensable for their functional and clonogenic death.
Caspase-8 promotes cell motility and calpain activity under nonapoptotic conditions.
Structural requirements for signal-induced target binding of FADD determined by functional reconstitution of FADD deficiency.
Role of membrane sphingomyelin and ceramide in platform formation for Fas-mediated apoptosis.
Caspase-8 serves both apoptotic and nonapoptotic roles.
Modifications and intracellular trafficking of FADD/MORT1 and caspase-8 after stimulation of T lymphocytes.
Apaf-1 and caspase-9 do not act as tumor suppressors in myc-induced lymphomagenesis or mouse embryo fibroblast transformation.
Zhong J, Reece EA, Yang P
Biochemical and biophysical research communications 2015 Nov 13;467(2):179-84
Biochemical and biophysical research communications 2015 Nov 13;467(2):179-84
NIK promotes tissue destruction independently of the alternative NF-κB pathway through TNFR1/RIP1-induced apoptosis.
Boutaffala L, Bertrand MJ, Remouchamps C, Seleznik G, Reisinger F, Janas M, Bénézech C, Fernandes MT, Marchetti S, Mair F, Ganeff C, Hupalowska A, Ricci JE, Becher B, Piette J, Knolle P, Caamano J, Vandenabeele P, Heikenwalder M, Dejardin E
Cell death and differentiation 2015 Dec;22(12):2020-33
Cell death and differentiation 2015 Dec;22(12):2020-33
Cellular-FLIP, Raji isoform (c-FLIP R) modulates cell death induction upon T-cell activation and infection.
Telieps T, Ewald F, Gereke M, Annemann M, Rauter Y, Schuster M, Ueffing N, von Smolinski D, Gruber AD, Bruder D, Schmitz I
European journal of immunology 2013 Jun;43(6):1499-510
European journal of immunology 2013 Jun;43(6):1499-510
AIM2/ASC triggers caspase-8-dependent apoptosis in Francisella-infected caspase-1-deficient macrophages.
Pierini R, Juruj C, Perret M, Jones CL, Mangeot P, Weiss DS, Henry T
Cell death and differentiation 2012 Oct;19(10):1709-21
Cell death and differentiation 2012 Oct;19(10):1709-21
Protease activity of procaspase-8 is essential for cell survival by inhibiting both apoptotic and nonapoptotic cell death dependent on receptor-interacting protein kinase 1 (RIP1) and RIP3.
Kikuchi M, Kuroki S, Kayama M, Sakaguchi S, Lee KK, Yonehara S
The Journal of biological chemistry 2012 Nov 30;287(49):41165-73
The Journal of biological chemistry 2012 Nov 30;287(49):41165-73
Mechanisms of necroptosis in T cells.
Ch'en IL, Tsau JS, Molkentin JD, Komatsu M, Hedrick SM
The Journal of experimental medicine 2011 Apr 11;208(4):633-41
The Journal of experimental medicine 2011 Apr 11;208(4):633-41
Apoptosis and non-inflammatory phagocytosis can be induced by mitochondrial damage without caspases.
van Delft MF, Smith DP, Lahoud MH, Huang DC, Adams JM
Cell death and differentiation 2010 May;17(5):821-32
Cell death and differentiation 2010 May;17(5):821-32
The death domain of FADD is essential for embryogenesis, lymphocyte development, and proliferation.
Imtiyaz HZ, Zhou X, Zhang H, Chen D, Hu T, Zhang J
The Journal of biological chemistry 2009 Apr 10;284(15):9917-26
The Journal of biological chemistry 2009 Apr 10;284(15):9917-26
Antigen-mediated T cell expansion regulated by parallel pathways of death.
Ch'en IL, Beisner DR, Degterev A, Lynch C, Yuan J, Hoffmann A, Hedrick SM
Proceedings of the National Academy of Sciences of the United States of America 2008 Nov 11;105(45):17463-8
Proceedings of the National Academy of Sciences of the United States of America 2008 Nov 11;105(45):17463-8
A cell-type-specific requirement for IFN regulatory factor 5 (IRF5) in Fas-induced apoptosis.
Couzinet A, Tamura K, Chen HM, Nishimura K, Wang Z, Morishita Y, Takeda K, Yagita H, Yanai H, Taniguchi T, Tamura T
Proceedings of the National Academy of Sciences of the United States of America 2008 Feb 19;105(7):2556-61
Proceedings of the National Academy of Sciences of the United States of America 2008 Feb 19;105(7):2556-61
Mutation of a self-processing site in caspase-8 compromises its apoptotic but not its nonapoptotic functions in bacterial artificial chromosome-transgenic mice.
Kang TB, Oh GS, Scandella E, Bolinger B, Ludewig B, Kovalenko A, Wallach D
Journal of immunology (Baltimore, Md. : 1950) 2008 Aug 15;181(4):2522-32
Journal of immunology (Baltimore, Md. : 1950) 2008 Aug 15;181(4):2522-32
Blockade of tumor necrosis factor-induced Bid cleavage by caspase-resistant Rb.
Huang X, Masselli A, Frisch SM, Hunton IC, Jiang Y, Wang JY
The Journal of biological chemistry 2007 Oct 5;282(40):29401-13
The Journal of biological chemistry 2007 Oct 5;282(40):29401-13
FLIP(L) protects neurons against in vivo ischemia and in vitro glucose deprivation-induced cell death.
Taoufik E, Valable S, Müller GJ, Roberts ML, Divoux D, Tinel A, Voulgari-Kokota A, Tseveleki V, Altruda F, Lassmann H, Petit E, Probert L
The Journal of neuroscience : the official journal of the Society for Neuroscience 2007 Jun 20;27(25):6633-46
The Journal of neuroscience : the official journal of the Society for Neuroscience 2007 Jun 20;27(25):6633-46
Loss of caspase-9 provides genetic evidence for the type I/II concept of CD95-mediated apoptosis.
Samraj AK, Keil E, Ueffing N, Schulze-Osthoff K, Schmitz I
The Journal of biological chemistry 2006 Oct 6;281(40):29652-9
The Journal of biological chemistry 2006 Oct 6;281(40):29652-9
Apaf-1 and caspase-9 are required for cytokine withdrawal-induced apoptosis of mast cells but dispensable for their functional and clonogenic death.
Marsden VS, Kaufmann T, O'reilly LA, Adams JM, Strasser A
Blood 2006 Mar 1;107(5):1872-7
Blood 2006 Mar 1;107(5):1872-7
Caspase-8 promotes cell motility and calpain activity under nonapoptotic conditions.
Helfer B, Boswell BC, Finlay D, Cipres A, Vuori K, Bong Kang T, Wallach D, Dorfleutner A, Lahti JM, Flynn DC, Frisch SM
Cancer research 2006 Apr 15;66(8):4273-8
Cancer research 2006 Apr 15;66(8):4273-8
Structural requirements for signal-induced target binding of FADD determined by functional reconstitution of FADD deficiency.
Imtiyaz HZ, Zhang Y, Zhang J
The Journal of biological chemistry 2005 Sep 9;280(36):31360-7
The Journal of biological chemistry 2005 Sep 9;280(36):31360-7
Role of membrane sphingomyelin and ceramide in platform formation for Fas-mediated apoptosis.
Miyaji M, Jin ZX, Yamaoka S, Amakawa R, Fukuhara S, Sato SB, Kobayashi T, Domae N, Mimori T, Bloom ET, Okazaki T, Umehara H
The Journal of experimental medicine 2005 Jul 18;202(2):249-59
The Journal of experimental medicine 2005 Jul 18;202(2):249-59
Caspase-8 serves both apoptotic and nonapoptotic roles.
Kang TB, Ben-Moshe T, Varfolomeev EE, Pewzner-Jung Y, Yogev N, Jurewicz A, Waisman A, Brenner O, Haffner R, Gustafsson E, Ramakrishnan P, Lapidot T, Wallach D
Journal of immunology (Baltimore, Md. : 1950) 2004 Sep 1;173(5):2976-84
Journal of immunology (Baltimore, Md. : 1950) 2004 Sep 1;173(5):2976-84
Modifications and intracellular trafficking of FADD/MORT1 and caspase-8 after stimulation of T lymphocytes.
O'Reilly LA, Divisekera U, Newton K, Scalzo K, Kataoka T, Puthalakath H, Ito M, Huang DC, Strasser A
Cell death and differentiation 2004 Jul;11(7):724-36
Cell death and differentiation 2004 Jul;11(7):724-36
Apaf-1 and caspase-9 do not act as tumor suppressors in myc-induced lymphomagenesis or mouse embryo fibroblast transformation.
Scott CL, Schuler M, Marsden VS, Egle A, Pellegrini M, Nesic D, Gerondakis S, Nutt SL, Green DR, Strasser A
The Journal of cell biology 2004 Jan 5;164(1):89-96
The Journal of cell biology 2004 Jan 5;164(1):89-96
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Supportive validation
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Flow cytometry of HEK 293T cells transfected with FLAG tagged mutated mouse caspase-8. Method: MAbs 1G12 and 3B10 both detect overexpressed FLAG-epitope-tagged pro-caspase-8 (active-site-mutant) by flow cytometry in permeabilized cells. As a positive control staining with a MAb to FLAG was performed. As a negative control an isotype control was employed.
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- MAbs 1G12 and 3B10 both detect pro-caspase-8 in MEFs from WT mice, but not in MEFs from caspase-8-/- mice. Several smaller bands detected in the caspase-8-/- MEFs, correspond to truncated forms of caspase-8 made in the caspase-8-/- mice since only exons 1 and 2 of mouse caspase-8 were deleted in these knock-out mice and not the region encoding the p18 subunit.Note: Extra bands marked by * are only seen in lysates from caspase-8-/- MEFs and not in lysates from any WT cell lines or mouse WT tissue. Both MAbs to caspase-8 (mouse) do not recognize human caspase-8, whereas endogenous caspase-8 can be efficiently detected in various mouse cell lines. Upon an apoptotic stimulus e.g. by cross-linked rhsFasL both MAbs to caspase-8 (mouse) do also recognize the cleaved active p18 subunit of mouse caspase-8 in addition to the caspase-8 precursor.
