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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- GTX101303 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX101303, RRID:AB_1949746
- Product name
- BLM antibody [C3], C-term
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references Microhomology-mediated End Joining and Homologous Recombination share the initial end resection step to repair DNA double-strand breaks in mammalian cells.
Truong LN, Li Y, Shi LZ, Hwang PY, He J, Wang H, Razavian N, Berns MW, Wu X
Proceedings of the National Academy of Sciences of the United States of America 2013 May 7;110(19):7720-5
Proceedings of the National Academy of Sciences of the United States of America 2013 May 7;110(19):7720-5
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Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate)? ? A: Jurkat ? B: Raji ? C: K562 ? D: NCI-H929 ? 5% SDS PAGE? ? GTX101303 diluted at 1:1000? ? The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate)? ? ? ? ? A: NIH-3T3 ? ? ? ? B: JC ? ? ? ? C: BCL-1 ? ? ? ? 5% SDS PAGE? ? ? ? ? GTX101303 diluted at 1:1000 ? ? ? ?? The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- BLM antibody [C3], C-term detects BLM protein at nucleus by immunofluorescent analysis.Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: BLM protein stained by BLM antibody [C3], C-term (GTX101303) diluted at 1:200.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using BLM(GTX101303) antibody at 1:100 dilution.
