MA5-27843

antibody from Invitrogen Antibodies

Targeting: ENY2 DC6, FLJ20480, Sus1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-27843

Provider

Invitrogen Antibodies

Product name

ENY2 Monoclonal Antibody (GT245)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Positive Control: U87-MG, SK-N-SH, IMR32

Antibody clone number

GT245

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ENY2 in A) U87-MG whole cell lysate, B) SK-N-SH whole cell lysate, C) IMR32 whole cell lysate using 30 µg of protein. Samples were separated with 15% SDS-PAGE and incubated with ENY2 monoclonal antibody (Product # MA5-27843) using a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using ENY2 Monoclonal Antibody (GT245) (Product # MA5-27843). Whole cell extract (30 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with ENY2 Monoclonal Antibody (GT245) (Product # MA5-27843) diluted at 1:1,000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using ENY2 Monoclonal Antibody (GT245) (Product # MA5-27843). Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with ENY2 Monoclonal Antibody (GT245) (Product # MA5-27843) diluted at 1:1,000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ENY2 antibody detects ENY2 protein at Nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde/PBS for 15 min. Green: ENY2 protein stained by ENY2 antibody (Product # MA5-27843) diluted at 1:500. Blue: Hoechst 33342 staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ENY2 Monoclonal Antibody (GT245) detects ENY2 protein at nucleus in rat testis by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. ENY2 Monoclonal Antibody (GT245) (Product # MA5-27843) diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.