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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
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Validation data
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- Product number
- PA5-29586 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- C1QA Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: human plasma, mouse spleen. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.18 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Knockdown of circulating C1 inhibitor induces neurovascular impairment, glial cell activation, neuroinflammation, and behavioral deficits.
Farfara D, Feierman E, Richards A, Revenko AS, MacLeod RA, Norris EH, Strickland S
Glia 2019 Jul;67(7):1359-1373
Glia 2019 Jul;67(7):1359-1373
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Complement C1qA using 50 µg mouse spleen lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a Complement C1qA polyclonal antibody (Product # PA5-29586) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Complement C1qA using 30 µg of HepG2 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a Complement C1qA polyclonal antibody (Product # PA5-29586) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of C1q was performed by separating 30 µg of human plasma by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a C1q Polyclonal Antibody (Product # PA5-29586) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using C1QA Polyclonal Antibody (Product # PA5-29586). Human plasma (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with C1QA Polyclonal Antibody (Product # PA5-29586) diluted at 1:10,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of C1q was performed in HeLa cells fixed in ice-cold MeOH for 5 min. Green: C1q Polyclonal Antibody (Product # PA5-29586) diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
