PA5-29586

antibody from Invitrogen Antibodies

Targeting: C1QA

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-29586

Provider

Invitrogen Antibodies

Product name

C1QA Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: human plasma, mouse spleen.

Concentration

0.96 mg/mL

References

Submitted references

Knockdown of circulating C1 inhibitor induces neurovascular impairment, glial cell activation, neuroinflammation, and behavioral deficits.

Farfara D, Feierman E, Richards A, Revenko AS, MacLeod RA, Norris EH, Strickland S
Glia 2019 Jul;67(7):1359-1373

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Complement C1qA using 50 µg mouse spleen lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a Complement C1qA polyclonal antibody (Product # PA5-29586) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Complement C1qA using 30 µg of HepG2 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a Complement C1qA polyclonal antibody (Product # PA5-29586) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of C1q was performed by separating 30 µg of human plasma by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a C1q Polyclonal Antibody (Product # PA5-29586) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using C1QA Polyclonal Antibody (Product # PA5-29586). Human plasma (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with C1QA Polyclonal Antibody (Product # PA5-29586) diluted at 1:10,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of C1q was performed in HeLa cells fixed in ice-cold MeOH for 5 min. Green: C1q Polyclonal Antibody (Product # PA5-29586) diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar = 10 µm.