HPA050553

antibody from Atlas Antibodies

Targeting: FMC1 C7orf55, HSPC268

Western blot (Enhanced validation)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA050553

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA050553, RRID:AB_2681173

Product name

Anti-FMC1

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

ELHFQAATYLCLLRSIRKHVALHQEFHGKGERSVE
ESAGLVGLKLPHQPGGKGWEP


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Western blot analysis in human cell lines HEK293 and U-251MG using Anti-C7orf55 antibody. Corresponding C7orf55 RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Western blot analysis in human cell lines HEK293 and U-251MG using Anti-FMC1 antibody. Corresponding FMC1 RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.

Orthogonal method

Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.

Show more

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line MCF7 shows localization to mitochondria.

Sample type

HUMAN

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.

Sample type

HUMAN