NBP1-88769
antibody from Novus Biologicals
Targeting: SNAP25
bA416N4.2, dJ1068F16.2, RIC-4, RIC4, SEC9, SNAP, SNAP-25
Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [3]
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Validation data
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- Product number
- NBP1-88769 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-88769, RRID:AB_11004084
- Product name
- Rabbit Polyclonal SNAP25 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human SNAP25 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Perturbation to Cholesterol at the Neuromuscular Junction Confers Botulinum Neurotoxin A Sensitivity to Neonatal Mice.
Novel pancreatic beta cell-specific proteins: antibody-based proteomics for identification of new biomarker candidates.
Immunohistochemical approach to the pathogenesis of clinical cases of bovine Herpesvirus type 5 infections.
Tissue profiling of the mammalian central nervous system using human antibody-based proteomics.
Thyagarajan B, Potian JG, McArdle JJ, Baskaran P
Toxicological sciences : an official journal of the Society of Toxicology 2017 Sep 1;159(1):179-188
Toxicological sciences : an official journal of the Society of Toxicology 2017 Sep 1;159(1):179-188
Novel pancreatic beta cell-specific proteins: antibody-based proteomics for identification of new biomarker candidates.
Lindskog C, Korsgren O, Pontén F, Eriksson JW, Johansson L, Danielsson A
Journal of proteomics 2012 May 17;75(9):2611-20
Journal of proteomics 2012 May 17;75(9):2611-20
Immunohistochemical approach to the pathogenesis of clinical cases of bovine Herpesvirus type 5 infections.
Cardoso TC, Ferrari HF, Garcia AF, Bregano LC, Andrade AL, Nogueira AH
Diagnostic pathology 2010 Sep 10;5:57
Diagnostic pathology 2010 Sep 10;5:57
Tissue profiling of the mammalian central nervous system using human antibody-based proteomics.
Mulder J, Björling E, Jonasson K, Wernérus H, Hober S, Hökfelt T, Uhlén M
Molecular & cellular proteomics : MCP 2009 Jul;8(7):1612-22
Molecular & cellular proteomics : MCP 2009 Jul;8(7):1612-22
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SNAP25 Antibody [NBP1-88769] - Analysis in mouse cerebral cortex tissue.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SNAP25 Antibody [NBP1-88769] - Analysis in human cell line SH-SY5Y.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SNAP25 Antibody [NBP1-88769] - Analysis in human cell lines U2OS and MCF-7 using anti-SNAP25 antibody. Corresponding SNAP25 RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SNAP25 Antibody [NBP1-88769] - Analysis in control (vector only transfected HEK293T lysate) and SNAP25 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SNAP25 Antibody [NBP1-88769] - Staining of human cerebral cortex shows high expression.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SNAP25 Antibody [NBP1-88769] - Staining of human pancreas shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SNAP25 Antibody [NBP1-88769] - Staining in human cerebral cortex and pancreas tissues using anti-SNAP25 antibody. Corresponding SNAP25 RNA-seq data are presented for the same tissues.