Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- TA324978 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal TEC Antibody (Center)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal TEC Antibody (Center)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- TEC
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- 2.0 mg/ml
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of anti-TEC Pab (Cat. #TA324978) in mouse liver tissue lysate. TEC (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- TEC Antibody (F190) (Cat. #TA324978) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the TEC antibody detected the TEC protein (arrow).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- IF image of Hela cell stained with TEC Antibody (Center)(Cat#TA324978).Hela cells were incubated with TEC primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7 units/ml).TEC immunoreactivity is localized to Cytoplasm significantly.
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- IF image of Hela cell stained with TEC Antibody (Center)(Cat#TA324978).Hela cells were incubated with TEC primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7 units/ml).TEC immunoreactivity is localized to Cytoplasm significantly.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
- Validation comment
- IHC
