NB100-68209
antibody from Novus Biologicals
Targeting: HCFC1
CFF, HCF-1, HCF1, HFC1, MGC70925, MRX3, PPP1R89, VCAF
Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NB100-68209 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-68209, RRID:AB_1108786
- Product name
- Rabbit Polyclonal Host Cell Factor 1/HCFC1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Interaction of DNA demethylase and histone methyltransferase upregulates Nrf2 in 5-fluorouracil-resistant colon cancer cells.
A transcriptional regulatory role of the THAP11-HCF-1 complex in colon cancer cell function.
The evolutionarily conserved longevity determinants HCF-1 and SIR-2.1/SIRT1 collaborate to regulate DAF-16/FOXO.
Kang KA, Piao MJ, Ryu YS, Kang HK, Chang WY, Keum YS, Hyun JW
Oncotarget 2016 Jun 28;7(26):40594-40620
Oncotarget 2016 Jun 28;7(26):40594-40620
A transcriptional regulatory role of the THAP11-HCF-1 complex in colon cancer cell function.
Parker JB, Palchaudhuri S, Yin H, Wei J, Chakravarti D
Molecular and cellular biology 2012 May;32(9):1654-70
Molecular and cellular biology 2012 May;32(9):1654-70
The evolutionarily conserved longevity determinants HCF-1 and SIR-2.1/SIRT1 collaborate to regulate DAF-16/FOXO.
Rizki G, Iwata TN, Li J, Riedel CG, Picard CL, Jan M, Murphy CT, Lee SS
PLoS genetics 2011 Sep;7(9):e1002235
PLoS genetics 2011 Sep;7(9):e1002235
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68209] - Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-HCF1 antibody NB100-68209 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. HCF1 was also immunoprecipitated by rabbit anti-HCF1 antibody NB100-68210, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).