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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
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- Product number
- AP08709PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP08709PU-N, RRID:AB_1975631
- Product name
- anti NMDAR2B pTyr1336
- Antibody type
- Polyclonal
- Antigen
- Phosphopeptide corresponding to amino acid residues surrounding the phospho-Tyr1336 of the NR2B subunit of the rat NMDA receptor.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
Submitted references Alcohol inhibition of the NMDA receptor function, long-term potentiation, and fear learning requires striatal-enriched protein tyrosine phosphatase.
Neurite degeneration induced by heme deficiency mediated via inhibition of NMDA receptor-dependent extracellular signal-regulated kinase 1/2 activation.
Hicklin TR, Wu PH, Radcliffe RA, Freund RK, Goebel-Goody SM, Correa PR, Proctor WR, Lombroso PJ, Browning MD
Proceedings of the National Academy of Sciences of the United States of America 2011 Apr 19;108(16):6650-5
Proceedings of the National Academy of Sciences of the United States of America 2011 Apr 19;108(16):6650-5
Neurite degeneration induced by heme deficiency mediated via inhibition of NMDA receptor-dependent extracellular signal-regulated kinase 1/2 activation.
Chernova T, Steinert JR, Guerin CJ, Nicotera P, Forsythe ID, Smith AG
The Journal of neuroscience : the official journal of the Society for Neuroscience 2007 Aug 8;27(32):8475-85
The Journal of neuroscience : the official journal of the Society for Neuroscience 2007 Aug 8;27(32):8475-85
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot of rat hippocampal lysate showing specific immunolabeling of the ~180k NR2B subunit of the NMDAR phosphorylated at Tyr1336 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the phospho-Tyr1336 NMDA NR2B antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- NR2B Tyr1336 and PSD95 Double-Label Immunostaining of 14 DIV Cortical Neurons.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunostaining of mouse dentate gyrus 48 hr post TMT treatment showing NR2B when phosphorylated at Tyr1336 in green and nuclei in blue. Photo Courtesy of Rob Wine.
