AP08708PU-N

antibody from Acris Antibodies GmbH

Targeting: GRIN2B GluN2B, NMDAR2B

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

AP08708PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP08708PU-N, RRID:AB_1975630

Product name

anti NMDAR2B pTyr1252

Antibody type

Polyclonal

Antigen

Phosphopeptide corresponding to amino acid residues surrounding the phospho-Tyr1252 of the NR2B subunit of the rat NMDA receptor.

Reactivity

Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Zebrafish

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

References

Submitted references

Alcohol inhibition of the NMDA receptor function, long-term potentiation, and fear learning requires striatal-enriched protein tyrosine phosphatase.

Hicklin TR, Wu PH, Radcliffe RA, Freund RK, Goebel-Goody SM, Correa PR, Proctor WR, Lombroso PJ, Browning MD
Proceedings of the National Academy of Sciences of the United States of America 2011 Apr 19;108(16):6650-5

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Figure 1. Western blot of rat hippocampal lysate showing specific immunolabeling of the ~180k NR2B subunit of the NMDAR phosphorylated at Tyr1252 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the phospho-Tyr1252 NMDA NR2B subunit antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.

Immunocytochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Figure 2. NR2B Tyr1252 and PSD95 Double-Label Immunostaining of 14 DIV Cortical Neurons.