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Immunocytochemistry
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Validations
- Flow cytometry [1]
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- Product number
- AF7204 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal Spi-B Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse Spi-B in direct ELISAs. In direct ELISAs, less than 2% cross-reactivity with recombinant human Spi-B is observed.
- Reactivity
- Mouse
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Osteoprotegerin-dependent M cell self-regulation balances gut infection and immunity.
The role of CSF1R-dependent macrophages in control of the intestinal stem-cell niche.
Development of intestinal M cells and follicle-associated epithelium is regulated by TRAF6-mediated NF-κB signaling.
c-Rel is dispensable for the differentiation and functional maturation of M cells in the follicle-associated epithelium.
Increased Abundance of M Cells in the Gut Epithelium Dramatically Enhances Oral Prion Disease Susceptibility.
Kimura S, Nakamura Y, Kobayashi N, Shiroguchi K, Kawakami E, Mutoh M, Takahashi-Iwanaga H, Yamada T, Hisamoto M, Nakamura M, Udagawa N, Sato S, Kaisho T, Iwanaga T, Hase K
Nature communications 2020 Jan 13;11(1):234
Nature communications 2020 Jan 13;11(1):234
The role of CSF1R-dependent macrophages in control of the intestinal stem-cell niche.
Sehgal A, Donaldson DS, Pridans C, Sauter KA, Hume DA, Mabbott NA
Nature communications 2018 Mar 28;9(1):1272
Nature communications 2018 Mar 28;9(1):1272
Development of intestinal M cells and follicle-associated epithelium is regulated by TRAF6-mediated NF-κB signaling.
Kanaya T, Sakakibara S, Jinnohara T, Hachisuka M, Tachibana N, Hidano S, Kobayashi T, Kimura S, Iwanaga T, Nakagawa T, Katsuno T, Kato N, Akiyama T, Sato T, Williams IR, Ohno H
The Journal of experimental medicine 2018 Feb 5;215(2):501-519
The Journal of experimental medicine 2018 Feb 5;215(2):501-519
c-Rel is dispensable for the differentiation and functional maturation of M cells in the follicle-associated epithelium.
Sehgal A, Kobayashi A, Donaldson DS, Mabbott NA
Immunobiology 2017 Feb;222(2):316-326
Immunobiology 2017 Feb;222(2):316-326
Increased Abundance of M Cells in the Gut Epithelium Dramatically Enhances Oral Prion Disease Susceptibility.
Donaldson DS, Sehgal A, Rios D, Williams IR, Mabbott NA
PLoS pathogens 2016 Dec;12(12):e1006075
PLoS pathogens 2016 Dec;12(12):e1006075
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of SPi-B in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Sheep Anti-Mouse Spi-B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7204) followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127) and Rat Anti-Mouse B220/CD45R PE-conjugated Monoclonal Antibody (Catalog # FAB1217P). Quadrant markers were set based on control antibody staining (Catalog # 5-001-A). To facilitate intracellular staining, cells were fixed with paraformadehyde and permeabilized with saponin.
