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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Western blot [2]
- ELISA [1]
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- Product number
- H00005295-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00005295-M01, RRID:AB_608992
- Product name
- Mouse Monoclonal PI 3-Kinase p85 alpha Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. PIK3R1 - phosphoinositide-3-kinase, regulatory subunit 1 (p85 alpha)
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Analysis of protein-protein interactions in cross-talk pathways reveals CRKL protein as a novel prognostic marker in hepatocellular carcinoma.
Liu CH, Chen TC, Chau GY, Jan YH, Chen CH, Hsu CN, Lin KT, Juang YL, Lu PJ, Cheng HC, Chen MH, Chang CF, Ting YS, Kao CY, Hsiao M, Huang CY
Molecular & cellular proteomics : MCP 2013 May;12(5):1335-49
Molecular & cellular proteomics : MCP 2013 May;12(5):1335-49
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PI 3-Kinase p85 alpha Antibody (3A10) [H00005295-M01] - Analysis of PIK3R1 expression in transfected 293T cell line by PIK3R1 monoclonal antibody (M01), clone 3A10.Lane 1: PIK3R1 transfected lysate (Predicted MW: 50 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PI 3-Kinase p85 alpha Antibody (3A10) [H00005295-M01] - PIK3R1 monoclonal antibody (M01), clone 3A10 Analysis of PIK3R1 expression in HeLa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: PI 3-Kinase p85 alpha Antibody (3A10) [H00005295-M01] - Detection limit for recombinant GST tagged PIK3R1 is approximately 0.1ng/ml as a capture antibody.
