60225-1-IG

antibody from Invitrogen Antibodies

Targeting: PIK3R1 GRB1, p85, p85-ALPHA

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

60225-1-IG

Provider

Invitrogen Antibodies

Product name

PI3K p85 alpha Monoclonal Antibody (4G3C11)

Antibody type

Monoclonal

Antigen

Other

Description

Immunogen sequence: RDTADGTFL VRDASTKMHG DYTLTLRKGG NNKLIKIFHR DGKYGFSDPL TFSSVVELIN HYRNESLAQY NPKLDVKLLY PVSKYQQDQV VKEDNIEAVG KKLHKYNTQF QEKSREYDRL YEEYTRTSQE IQMKRTAIEA FNETIKIFEE QCQTQERYSK EYIEKFKREG NEKEIQRIMH NYDKLKSRIS EIIDSRRRLE EDLKKQAAEY REIDKRMNSI KPDLIQLRKT RDQYLMWLTQ KGVRQKKLNE WLGNENTEDQ YSLVEDDEDL PHHDEKTWNV GSSNRNKAEN LLRGKRDGTF LVRESSKQGC YACSVVVDGE VKHCVINKTA TGYGFAEPYN LYSSLKELVL HYQHTSLVQH NDSLNVTLAY PVYAQQRR (78-454 aa encoded by BC030815 )

Reactivity

Human, Mouse, Rat, Porcine

Host

Mouse

Isotype

IgG

Antibody clone number

4G3C11

Vial size

150 µL

Concentration

1.5 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

LNCaP cells were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3K p85 alpha antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Pig brain tissue were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3K p85 (alpha) antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse brain tissue were subjected to SDS PAGE followed by western blot with 60225-1-Ig (PI3K p85 (alpha) antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Various lysates were subjected to SDS PAGE followed by western blot with 60225-1-IG (PI3K p85 alpha antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of RAW 264.7 cells using 60225-1-Ig (PI3K p85 (alpha) antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( -20°C Ethanol ) fixed HeLa cells using 60225-1-IG (PI3K p85 (alpha) antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using 60225-1-Ig (PI3K p85 (alpha) antibody at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using 60225-1-Ig (PI3K p85 (alpha) antibody at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human skeletal muscle tissue slide using 60225-1-Ig (PI3K p85 (alpha) antibody at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human skeletal muscle tissue slide using 60225-1-Ig (PI3K p85 (alpha) antibody at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 60225-1-Ig (PI3K p85 (alpha) antibody at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 60225-1-Ig (PI3K p85 (alpha) antibody at dilution of 1:200 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using (Product # 60225-1-IG) (PI3 Kinase p85 Alpha antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human prostate cancer slide using 60225-1-IG ( PIK3R1 Antibody) at dilution of 1:50.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human prostate cancer slide using 60225-1-IG ( PIK3R1 Antibody) at dilution of 1:50.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-PI3K p85 (alpha) (IP: 60225-1-IG, 5 µg; Detection: 60225-1-IG 1:500) with RAW 264.7 cells lysate 1480 µg.