Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- BS-0128R - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PI3 kinase p85 alpha subunit Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Rat brain lysates probed with Anti PI3K/PI3 kinase p85 alpha subunit Polyclonal Antibody, Unconjugated (bs-0128R) at 1:200 in 4°C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90 min in 37°C. Predicted band 80 kD. Observed band size: 85 kD.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Rat brain lysates probed with Anti PI3K/PI3 kinase p85 alpha subunit Polyclonal Antibody, Unconjugated (bs-0128R) at 1:200 in 4°C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90 min in 37°C. Predicted band 80 kD. Observed band size: 85 kD.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HepG2 cells were stained with PI3 kinase p85 alpha subunit Polyclonal Antibody, Unconjugated(bs-0128R) at 1:500 in PBS and incubated for two hours at 37°C followed by Goat Anti-Rabbit IgG (H+L) Cy3 conjugated secondary antibody. DAPI staining of the nucleus was done and then detected.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin embedded rat brain labeled with Anti-PI3K/PI3 kinase p85 alpha subunit Polyclonal Antibody, Unconjugated (bs-0128R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- H9C2 cells probed with Rabbit Anti-PI3 kinase p85 alpha subunit Polyclonal Antibody (bs-0128R) at 1:100 for 30 minutes followed by incubation with Goat Anti-Rabbit IgG FITC conjugated secondary at 1:100 (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).