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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [6]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- GTX111068 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX111068, RRID:AB_11177966
- Product name
- PI3 kinase p85 alpha antibody [N2C1], Internal
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Jiao-tai-wan Up-regulates Hypothalamic and Peripheral Circadian Clock Gene Cryptochrome and Activates PI3K/AKT Signaling in Partially Sleep-deprived Rats.
The Inhibition of microRNA-128 on IGF-1-Activating mTOR Signaling Involves in Temozolomide-Induced Glioma Cell Apoptotic Death.
The Synthetic β-Nitrostyrene Derivative CYT-Rx20 Inhibits Esophageal Tumor Growth and Metastasis via PI3K/AKT and STAT3 Pathways.
Huang WY, Zou X, Lu FE, Su H, Zhang C, Ren YL, Fang K, Xu LJ, Wang KF, Chen QJ, Dong H
Current medical science 2018 Aug;38(4):704-713
Current medical science 2018 Aug;38(4):704-713
The Inhibition of microRNA-128 on IGF-1-Activating mTOR Signaling Involves in Temozolomide-Induced Glioma Cell Apoptotic Death.
Chen PH, Cheng CH, Shih CM, Ho KH, Lin CW, Lee CC, Liu AJ, Chang CK, Chen KC
PloS one 2016;11(11):e0167096
PloS one 2016;11(11):e0167096
The Synthetic β-Nitrostyrene Derivative CYT-Rx20 Inhibits Esophageal Tumor Growth and Metastasis via PI3K/AKT and STAT3 Pathways.
Chiu WC, Lee YC, Su YH, Wang YY, Tsai CH, Hou YA, Wang CH, Huang YF, Huang CJ, Chou SH, Hsieh PW, Yuan SF
PloS one 2016;11(11):e0166453
PloS one 2016;11(11):e0166453
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: Jurkat B: K562 C: THP-1 7.5% SDS PAGE GTX111068 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: BCL-1 7.5% SDS PAGE GTX111068 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PIK3R1 antibody detects PIK3R1 protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PIK3R1 antibody (GTX111068) diluted at 1:1000.
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p85 alpha antibody [N2C1], Internal (GTX111068) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p85 alpha antibody [N2C1], Internal (GTX111068) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p85 alpha antibody [N2C1], Internal (GTX111068) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PI3 kinase p85 alpha antibody [N2C1], Internal immunoprecipitates PI3 kinase p85 alpha protein in IP experiments.IP samples: Jurkat whole cell extractA. Control with 5 £gg of preimmune Rabbit IgGB. Immunoprecipitation of PI3 kinase p85 alpha protein by 5 £gg PI3 kinase p85 alpha antibody [N2C1], Internal (GTX111068)10 % SDS-PAGEThe immunoprecipitated PI3 kinase p85 alpha protein was detected by PI3 kinase p85 alpha antibody [N2C1], Internal (GTX111068) diluted at 1:500.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PI3 kinase p85 alpha antibody [N2C1], Internal detects PIK3R1 protein at cytoplasm in mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. PI3 kinase p85 alpha antibody [N2C1], Internal (GTX111068) diluted at 1:500.
