Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- ABIN1881283 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Epidermal Growth Factor Receptor (EGFR) (pTyr1069) antibody
- Antibody type
- Polyclonal
- Antigen
- This EGFR Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y1069 of human EGFR.
- Description
- This antibody is purified through a protein A column, followed by peptide affinity purification. Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- pTyr1069
- Antibody clone number
- RB11167
- Vial size
- 400 μL
- Concentration
- 0.42 mg/mL
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Supportive validation
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- antibodies-online (provider)
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- Experimental details
- (LEFT)Dot blot analysis of Phospho-EGFR-Y1069 polyclonal antibody (ABIN1881283) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentration was 0.5 μg per mL. P-Pab: phospho-antibody, P-Peptide: phospho-peptide, NP-Peptide: non-phospho-peptide. (RIGHT)Western blot analysis of EGFRY (arrow) in Hela cell lysates, either noninduced (Lane 1) or induced with EGF (Lane 2).