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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- IC3570F - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal EGFR Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human EGF R phosphorylated at Y1068 in flow cytometry.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.
Submitted references Loss of N-Acetylgalactosaminyltransferase-4 Orchestrates Oncogenic MicroRNA-9 in Hepatocellular Carcinoma.
Liu Y, Liu H, Yang L, Wu Q, Liu W, Fu Q, Zhang W, Zhang H, Xu J, Gu J
The Journal of biological chemistry 2017 Feb 24;292(8):3186-3200
The Journal of biological chemistry 2017 Feb 24;292(8):3186-3200
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Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of EGF R/ErbB1 in A431 Human Cell Line by Flow Cytometry. A431 human epithelial carcinoma cell line treated with recombinant human EGF (Catalog # 236-EG) was stained with Mouse Anti-Human Phospho-EGF R/ErbB1 (Y1068) Fluorescein-conjugated Monoclonal Antibody (Catalog # IC3570F, filled histogram) or isotype control antibody (Catalog # IC003F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
