GTX10643

antibody from GeneTex

Targeting: EGFR ERBB, ERBB1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

GTX10643

Provider

GeneTex

Proper citation

GeneTex Cat#GTX10643, RRID:AB_379815

Product name

EGFR (phospho Tyr1148) antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse

Host

Rabbit

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Peptide Competition. Extracts of A431 cells unstimulated (1) or stimulated with 200 ng/mL EGF (2-5) for 15 minutes were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was blocked with a 5% BSA-TBST buffer for one hour at room temperature, then incubated with the EGFR (phospho Tyr1148) antibody for two hours at room temperature in a 1% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), non-phosphopeptide corresponding to the phosphopeptide immunogen (3), a generic phosphotyrosine-containing peptide (4), or the phosphopeptide immunogen (5). The data show that only the phosphopeptide corresponding to EGFR (phospho Tyr1148) blocks the signal, demonstrating the specificity of the antibody. The data also show the induction of EGFR Tyr1148 phosphorylation by the addition of EGF to this cell system.