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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- GTX25644 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX25644, RRID:AB_380620
- Product name
- EGFR (phospho Tyr1068) antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Rabbit
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- Extracts of A431 cells unstimulated (1) or stimulated with 200 ng/mL EGF for 15 minutes (2-5), were probed with the EGFR [pY1068] antibody following prior incubation with: no peptide (1 and 2), the non-phosphopeptide corresponding to the phosphopeptide immunogen (3), a generic phosphotyrosine-containing peptide (4) or the phosphopeptide immunogen (5). The data show that only the phosphopeptide corresponding to EGFR [pY1068] blocks the antibody signal, demonstrating the specificity of the antibody. The data also show the induction of EGFR [pY1068] phosphorylation by the addition of EGF to this cell system.
