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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB2075 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB2075, RRID:AB_1574560
- Product name
- EGFR polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of EGFR.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Tyrosine phosphorylation of the beta2 subunit of clathrin adaptor complex AP-2 reveals the role of a di-leucine motif in the epidermal growth factor receptor trafficking.
Aldosterone stimulates epidermal growth factor receptor expression.
Distinct domains in the adenovirus E3 RIDalpha protein are required for degradation of Fas and the epidermal growth factor receptor.
Huang F, Jiang X, Sorkin A
The Journal of biological chemistry 2003 Oct 31;278(44):43411-7
The Journal of biological chemistry 2003 Oct 31;278(44):43411-7
Aldosterone stimulates epidermal growth factor receptor expression.
Krug AW, Grossmann C, Schuster C, Freudinger R, Mildenberger S, Govindan MV, Gekle M
The Journal of biological chemistry 2003 Oct 31;278(44):43060-6
The Journal of biological chemistry 2003 Oct 31;278(44):43060-6
Distinct domains in the adenovirus E3 RIDalpha protein are required for degradation of Fas and the epidermal growth factor receptor.
Zanardi TA, Yei S, Lichtenstein DL, Tollefson AE, Wold WS
Journal of virology 2003 Nov;77(21):11685-96
Journal of virology 2003 Nov;77(21):11685-96
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of EGFR polyclonal antibody (Cat # PAB2075) in ZR-75-1 cell lysate. EGFR (arrow) was detected using purified Polyclonal antibody.Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with EGFR polyclonal antibody (Cat # PAB2075) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
