PAB5331

antibody from Abnova Corporation

Targeting: EGFR ERBB, ERBB1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB5331

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB5331, RRID:AB_1574577

Product name

EGFR polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of EGFR.

Storage

Store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Ca2+/calmodulin-dependent kinase II phosphorylates the epidermal growth factor receptor on multiple sites in the cytoplasmic tail and serine 744 within the kinase domain to regulate signal generation.

Feinmesser RL, Wicks SJ, Taverner CJ, Chantry A
The Journal of biological chemistry 1999 Jun 4;274(23):16168-73

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Hydrogen peroxide preferentially enhances the tyrosine phosphorylation of epidermal growth factor receptor.

Gamou S, Shimizu N
FEBS letters 1995 Jan 3;357(2):161-4

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Calphostin-C stimulates epidermal growth factor receptor phosphorylation and internalization via light-dependent mechanism.

Gamou S, Shimizu N
Journal of cellular physiology 1994 Jan;158(1):151-9

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of the extracts from SK-OV-3 cells untreated or treated with EGF using EGFR polyclonal antibody (Cat # PAB5331) .

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using EGFR polyclonal antibody (Cat # PAB5331) .

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)