Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB5331 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB5331, RRID:AB_1574577
- Product name
- EGFR polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of EGFR.
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Ca2+/calmodulin-dependent kinase II phosphorylates the epidermal growth factor receptor on multiple sites in the cytoplasmic tail and serine 744 within the kinase domain to regulate signal generation.
Hydrogen peroxide preferentially enhances the tyrosine phosphorylation of epidermal growth factor receptor.
Calphostin-C stimulates epidermal growth factor receptor phosphorylation and internalization via light-dependent mechanism.
Feinmesser RL, Wicks SJ, Taverner CJ, Chantry A
The Journal of biological chemistry 1999 Jun 4;274(23):16168-73
The Journal of biological chemistry 1999 Jun 4;274(23):16168-73
Hydrogen peroxide preferentially enhances the tyrosine phosphorylation of epidermal growth factor receptor.
Gamou S, Shimizu N
FEBS letters 1995 Jan 3;357(2):161-4
FEBS letters 1995 Jan 3;357(2):161-4
Calphostin-C stimulates epidermal growth factor receptor phosphorylation and internalization via light-dependent mechanism.
Gamou S, Shimizu N
Journal of cellular physiology 1994 Jan;158(1):151-9
Journal of cellular physiology 1994 Jan;158(1):151-9
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of the extracts from SK-OV-3 cells untreated or treated with EGF using EGFR polyclonal antibody (Cat # PAB5331) .
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using EGFR polyclonal antibody (Cat # PAB5331) .
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)