PAB7901

antibody from Abnova Corporation

Targeting: EGFR ERBB, ERBB1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB7901

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB7901, RRID:AB_1707715

Product name

EGFR (phospho S1142) polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic phosphopeptide of EGFR.

Storage

Store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Systematic analysis of the epidermal growth factor receptor by mass spectrometry reveals stimulation-dependent multisite phosphorylation.

Boeri Erba E, Bergatto E, Cabodi S, Silengo L, Tarone G, Defilippi P, Jensen ON
Molecular & cellular proteomics : MCP 2005 Aug;4(8):1107-21

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The EGF receptor: a nexus for trafficking and signaling.

Carpenter G
BioEssays : news and reviews in molecular, cellular and developmental biology 2000 Aug;22(8):697-707

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot of human A-431 cells treated with Calyculin A (100 nM) for 30 min. Blot lanes were untreated (lane 1) or treated with lambda phosphatase (lane 2) then were probed with EGFR (phospho S1142) polyclonal antibody (Cat # PAB7901).

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunocytochemical labeling in A-431 cells untreated (1) or treated with Calyculin A (50 nM) (2) for 15 min. Cells were labeled with EGFR (phospho S1142) polyclonal antibody (Cat # PAB7901). The specificity of labeling was demonstrated with its respective blocking peptides EGFR (phospho S1142) polyclonal antibody (Cat # PAB7901) (3).

Validation comment

Immunofluorescence