PAB8085

antibody from Abnova Corporation

Targeting: EGFR ERBB, ERBB1

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB8085

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB8085, RRID:AB_1707743

Product name

EGFR (phospho Y1172) polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic phosphopeptide of EGFR.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

A basic peptide within the juxtamembrane region is required for EGF receptor dimerization.

Aifa S, Aydin J, Nordvall G, Lundström I, Svensson SP, Hermanson O
Experimental cell research 2005 Jan 1;302(1):108-14

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EGF signalling amplification induced by dynamic clustering of EGFR.

Ichinose J, Murata M, Yanagida T, Sako Y
Biochemical and biophysical research communications 2004 Nov 19;324(3):1143-9

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Signalling by the type 1 insulin-like growth factor receptor: interplay with the epidermal growth factor receptor.

Adams TE, McKern NM, Ward CW
Growth factors (Chur, Switzerland) 2004 Jun;22(2):89-95

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis using EGFR (pY1172) polyclonal antibody (Cat # PAB8085) to detect EGFR in A-431 cell lysate, and A-431 cell treated with EGF 25 ng/ml for 15 or 30 min. Loading 50 ug total protein each well, and EGFR (pY1172) polyclonal antibody were diluted 1:1000.

Other assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Dot blot analysis of EGFR (phospho Y1172) polyclonal antibody (Cat # PAB8085) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 ug/mL.

Validation comment

Dot Blot (Peptide)