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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
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- Product number
- MAB6476 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB6476, RRID:AB_10677064
- Product name
- EGFR (phospho Y992) monoclonal antibody, clone EM-12
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against synthetic peptide of EGFR.
- Isotype
- IgG
- Antibody clone number
- EM-12
- Storage
- Store at 4°C. Do not freeze.Aliquot to avoid repeated freezing and thawing.
Submitted references Alterations in genes of the EGFR signaling pathway and their relationship to EGFR tyrosine kinase inhibitor sensitivity in lung cancer cell lines.
Epidermal growth factor receptor activation protects gastric epithelial cells from Helicobacter pylori-induced apoptosis.
EGFR-dependent migration of glial cells is mediated by reorganisation of N-cadherin.
Gandhi J, Zhang J, Xie Y, Soh J, Shigematsu H, Zhang W, Yamamoto H, Peyton M, Girard L, Lockwood WW, Lam WL, Varella-Garcia M, Minna JD, Gazdar AF
PloS one 2009;4(2):e4576
PloS one 2009;4(2):e4576
Epidermal growth factor receptor activation protects gastric epithelial cells from Helicobacter pylori-induced apoptosis.
Yan F, Cao H, Chaturvedi R, Krishna U, Hobbs SS, Dempsey PJ, Peek RM Jr, Cover TL, Washington MK, Wilson KT, Polk DB
Gastroenterology 2009 Apr;136(4):1297-1307, e1-3
Gastroenterology 2009 Apr;136(4):1297-1307, e1-3
EGFR-dependent migration of glial cells is mediated by reorganisation of N-cadherin.
Rappl A, Piontek G, Schlegel J
Journal of cell science 2008 Dec 15;121(Pt 24):4089-97
Journal of cell science 2008 Dec 15;121(Pt 24):4089-97
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blotting analysis of EGFR (phospho-Tyr992) by EGFR (phospho Y992) monoclonal antibody, clone EM-12 (Cat # MAB6476) in EGF-treated A-431 (Lane 1), CALU-3 (Lane 2), MCF-7 (Lane 3), Jurkat (Lane 4) and Ramos (Lane 5) cell lines (reduced conditions).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of EGFR from EGF-treated A-431 cells by EGFR (phospho Y992) monoclonal antibody, clone EM-12 (Cat # MAB6476) (Lane 1), EGFR (phospho Y1173) monoclonal antibody, clone EM-13 (Cat # MAB6475) (Lane 2). The precipitates were immunoblotted with a commercial anti-EGFR polyclonal and goat anti-rabbit-HRP.
- Validation comment
- Immunoprecipitation
