NB100-596

antibody from Novus Biologicals

Targeting: EGFR ERBB, ERBB1

Immunoprecipitation

Antibody data

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Validation data

EGFR protein structure - NB100-596 shown in red.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: EGFR Antibody [NB100-596] - EGF R/ErbB1 Antibody [NB100-596] - Whole cell lysate (50 ug) from 293T and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody:Affinity purified rabbit anti-EGFR antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: EGFR Antibody [NB100-596] - Detection of Human EGFR on HeLa whole cell lysate using NB100-596.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: EGFR Antibody [NB100-596] - Detection of Human EGFR by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EGFR antibody NB100-596 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: EGFR Antibody [NB100-596] - NPM1 knockdown decreases EGF expression. NPM1 controls activation of the EGF/EGFR pathway downstream effectors. Proteins, extracted from shScr and shNPM1 LNCaP cells cultured in RPMI 1640 10%FBS, were electrophoresed by SDS-PAGE. Transferred membranes were immunoblotted with indicated antibodies. Histograms show the band quantification reported to the beta-actin level. Blots are representative of three independent experiments with consistent results. Data are representative of at least three independent experiments and are expressed as the mean +/- SD. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0096293), licensed under a CC-BY licence.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: EGFR Antibody [NB100-596] - NPM1 knockdown in prostate cancer cells reduces proliferation and migration capacities by inhibiting the EGF/EGFR pathway activity.(a) NPM1 down-regulation inhibits EGF induced ERK1/2 pathway activity. shScr and shNPM1 LNCaP cells were treated with 100 nM EGF and phosphorylation of the EGF/EGFR pathway effectors was analyzed using Western Blotting. Histograms show the band quantification reported to the beta-actin level. The blot is representative of three independent experiments with consistent results. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0096293), licensed under a CC-BY licence.