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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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- Product number
- GTX628888 - Provider product page
- Provider
- GeneTex
- Product name
- EGFR antibody [GT461]
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
Submitted references A high-throughput pipeline for validation of antibodies.
Sikorski K, Mehta A, Inngjerdingen M, Thakor F, Kling S, Kalina T, Nyman TA, Stensland ME, Zhou W, de Souza GA, Holden L, Stuchly J, Templin M, Lund-Johansen F
Nature methods 2018 Nov;15(11):909-912
Nature methods 2018 Nov;15(11):909-912
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Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) A431 whole cell extracts (15 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [GT461] (GTX628888) diluted at 1:8000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- EGFR antibody [GT461] detects EGFR protein by Western blot analysis.A. 30 ?g A431 whole cell lysate/extract5 % SDS-PAGEEGFR antibody [GT461] (GTX628888) dilution: 1:10000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) A431 whole cell extracts (15 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [GT461] (GTX628888) diluted at 1:8000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- EGFR antibody [GT461] detects EGFR protein at cell membrane by immunofluorescent analysis.Sample: A431 cells were fixed in 4% paraformaldehyde for 10 min.Green: EGFR protein stained by EGFR antibody [GT461] (GTX628888) diluted at 1:300.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of EGFR protein from A431 whole cell extracts using 5 £gg of EGFR antibody [GT461] (GTX628888).Western blot analysis was performed using EGFR antibody [GT461] (GTX628888).EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.
