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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00000012-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00000012-M01, RRID:AB_425281
- Product name
- SERPINA3 monoclonal antibody (M01), clone 1E6
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant SERPINA3.
- Antigen sequence
PNSPLDEENLTQENQDRGTHVDLGLASANVDFAFS
LYKQLVLKAPDKNVIFSPLSISTALAFLSLGAHNT
TLTEILKGLKFNLTETSEAEIHQSFQHLLRTLNQS
SDELQLSMGNAMFVKEQLSLLDRFTEDAKRLYGSE
AFATDFQDSAAAKKLINDYVKNGTRGKITDLIKDL
DSQTMMVLVNYIFFKAKWEMPFDPQDTHQSRFYLS
KKKWVMVPMMSLHHLTIPYFRDEELSCTVVELKYT
GNASALFILPDQDKMEEVEAMLLPETLKRWRDSLE
FREIGELYLPKFSISRDYNLNDILLQLGIEEAFTS
KADLSGITGARNLAVSQVVHKAVLDVFEEGTEASA
ATAVKITLLSALVETRTIVRFNRPFLMIIVPTDTQ
NIFFMSKVTNPKQA- Isotype
- IgG
- Antibody clone number
- 1E6
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of SERPINA3 expression in transfected 293T cell line by SERPINA3 monoclonal antibody (M01), clone 1E6.Lane 1: SERPINA3 transfected lysate(47.7 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged SERPINA3 is 1 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of SERPINA3 transfected lysate using anti-SERPINA3 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with SERPINA3 MaxPab rabbit polyclonal antibody.
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to SERPINA3 on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma. [antibody concentration 1 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
