Explore
Validate
Learn
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- H00000012-M02 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00000012-M02, RRID:AB_1137029
- Product name
- SERPINA3 monoclonal antibody (M02), clone 1C10
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full-length recombinant SERPINA3.
- Antigen sequence
PNSPLDEENLTQENQDRGTHVDLGLASANVDFAFS
LYKQLVLKAPDKNVIFSPLSISTALAFLSLGAHNT
TLTEILKGLKFNLTETSEAEIHQSFQHLLRTLNQS
SDELQLSMGNAMFVKEQLSLLDRFTEDAKRLYGSE
AFATDFQDSAAAKKLINDYVKNGTRGKITDLIKDL
DSQTMMVLVNYIFFKAKWEMPFDPQDTHQSRFYLS
KKKWVMVPMMSLHHLTIPYFRDEELSCTVVELKYT
GNASALFILPDQDKMEEVEAMLLPETLKRWRDSLE
FREIGELYLPKFSISRDYNLNDILLQLGIEEAFTS
KADLSGITGARNLAVSQVVHKAVLDVFEEGTEASA
ATAVKITLLSALVETRTIVRFNRPFLMIIVPTDTQ
NIFFMSKVTNPKQA- Isotype
- IgG
- Antibody clone number
- 1C10
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Whole genome RNA expression profiling of endoscopic biliary brushings provides data suitable for biomarker discovery in cholangiocarcinoma.
Chapman MH, Tidswell R, Dooley JS, Sandanayake NS, Cerec V, Deheragoda M, Lee AJ, Swanton C, Andreola F, Pereira SP
Journal of hepatology 2012 Apr;56(4):877-85
Journal of hepatology 2012 Apr;56(4):877-85
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged SERPINA3 is approximately 3ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to SERPINA3 on HeLa cell . [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to SERPINA3 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
