NBP1-44261

antibody from Novus Biologicals

Targeting: PIP4K2B PIP5K2B, PIP5KIIB, PIP5KIIbeta

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

NBP1-44261

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-44261, RRID:AB_2164568

Product name

Rabbit Polyclonal PIP5K2B Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. This antibody is specific to PIP5K2B

Reactivity

Human, Mouse, Rat, Chicken/Avian, Simian

Host

Rabbit

Isotype

IgG

Vial size

0.1 mg

Concentration

0.65 mg/ml

Storage

Store at -20C. Avoid freeze-thaw cycles.

References

Submitted references

Type II phosphatidylinositol 4-kinase beta is a cytosolic and peripheral membrane protein that is recruited to the plasma membrane and activated by Rac-GTP.

Wei YJ, Sun HQ, Yamamoto M, Wlodarski P, Kunii K, Martinez M, Barylko B, Albanesi JP, Yin HL
The Journal of biological chemistry 2002 Nov 29;277(48):46586-93

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Characterization of type II phosphatidylinositol 4-kinase isoforms reveals association of the enzymes with endosomal vesicular compartments.

Balla A, Tuymetova G, Barshishat M, Geiszt M, Balla T
The Journal of biological chemistry 2002 May 31;277(22):20041-50

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: PIP5K2B Antibody [NBP1-44261] - Lane 1: Mouse Brain homogenate samples. Load: 10 ug per lane. Primary antibody: PIP5K2B antibody at 0.2ug/mL for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:40,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 54 kDa for PIP5K2B. Other band(s): None.