Antibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Immunohistochemistry [2]
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Validation data
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- Product number
- NBP1-46568 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-46568, RRID:AB_10009845
- Product name
- Rabbit Polyclonal Connexin 40/GJA5 Antibody
- Antibody type
- Polyclonal
- Description
- Unpurified. Specific for Connexin-40.
- Reactivity
- Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- LYOPH
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Connexin 40/GJA5 Antibody [NBP1-46568] - IHC on paraffin sections of rat kidney tissue using Rabbit antibody to Connexin 40. HIER: 1 mM EDTA, pH 8 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer from Leica following manufactures instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer. Sections were counterstained with Harris Hematoxylin.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Connexin 40/GJA5 Antibody [NBP1-46568] - IHC on paraffin sections of rat kidney tissue using Rabbit antibody to Connexin 40. HIER: 1 mM EDTA, pH 8 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer from Leica following manufactures instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer. Sections were counterstained with Harris Hematoxylin.