- 37-8900 - Invitrogen Antibodies GJA5 antibody

Submitted references Novel Blood Vascular Endothelial Subtype-Specific Markers in Human Skin Unearthed by Single-Cell Transcriptomic Profiling.

He Y, Tacconi C, Dieterich LC, Kim J, Restivo G, Gousopoulos E, Lindenblatt N, Levesque MP, Claassen M, Detmar M
Cells 2022 Mar 25;11(7)

Cellular and molecular landscape of mammalian sinoatrial node revealed by single-cell RNA sequencing.

Liang D, Xue J, Geng L, Zhou L, Lv B, Zeng Q, Xiong K, Zhou H, Xie D, Zhang F, Liu J, Liu Y, Li L, Yang J, Xue Z, Chen YH
Nature communications 2021 Jan 12;12(1):287

In vitro generation of functional murine heart organoids via FGF4 and extracellular matrix.

Lee J, Sutani A, Kaneko R, Takeuchi J, Sasano T, Kohda T, Ihara K, Takahashi K, Yamazoe M, Morio T, Furukawa T, Ishino F
Nature communications 2020 Sep 3;11(1):4283

ZO-1 Regulates Intercalated Disc Composition and Atrioventricular Node Conduction.

Dai W, Nadadur RD, Brennan JA, Smith HL, Shen KM, Gadek M, Laforest B, Wang M, Gemel J, Li Y, Zhang J, Ziman BD, Yan J, Ai X, Beyer EC, Lakata EG, Kasthuri N, Efimov IR, Broman MT, Moskowitz IP, Shen L, Weber CR
Circulation research 2020 Jul 3;127(2):e28-e43

Probing Endothelial Cell Mechanics Through Connexin 43 Disruption.

Islam MM, Steward RL Jr
Experimental mechanics 2019 Mar;59(3):327-336

Single-cell transcriptomics reveal the dynamic of haematopoietic stem cell production in the aorta.

Baron CS, Kester L, Klaus A, Boisset JC, Thambyrajah R, Yvernogeau L, Kouskoff V, Lacaud G, van Oudenaarden A, Robin C
Nature communications 2018 Jun 28;9(1):2517

Re-evaluation of connexins associated with motoneurons in rodent spinal cord, sexually dimorphic motor nuclei and trigeminal motor nucleus.

Bautista W, Rash JE, Vanderpool KG, Yasumura T, Nagy JI
The European journal of neuroscience 2014 Mar;39(5):757-70

Connexins and M3 muscarinic receptors contribute to heterogeneous Ca(2+) signaling in mouse aortic endothelium.

Boittin FX, Alonso F, Le Gal L, Allagnat F, Bény JL, Haefliger JA
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2013;31(1):166-78

Intercellular calcium waves in primary cultured rat mesenteric smooth muscle cells are mediated by connexin43.

Halidi N, Alonso F, Burt JM, Bény JL, Haefliger JA, Meister JJ
Cell communication & adhesion 2012 Apr;19(2):25-37

Loss of connexin40 is associated with decreased endothelium-dependent relaxations and eNOS levels in the mouse aorta.

Alonso F, Boittin FX, Bény JL, Haefliger JA
American journal of physiology. Heart and circulatory physiology 2010 Nov;299(5):H1365-73

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Supportive validation

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Experimental details: Western blot analysis of recombinant human p19 using Ms anti-p19INK4d (Product # 37-8700).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunohistochemistry analysis of Connexin 40 showing staining in the membrane of paraffin-embedded human lung adenocarcinoma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Connexin 40 monoclonal antibody (Product # 37-8900) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 5 Evaluation of the cell components of heart organoid (HO)s. a Immunofluorescent staining to detect cTnT (a CM marker, green)/alphaSMA (an SM marker, red) and b PECAM (an EC marker, red) in the HO and E10.5 heart cryosections. Expression of cTnT and alphaSMA was observed in the HOs ( n = 7 out of n = 9, two experiments). Also, segregated lining expression of PECAM (red) was detected in the HOs (over n = 9, two experiments). Scale bar: 100 mum. c - f Immunostaining to detect c GATA4 (red)/cTnT (green), d Cx43 (red)/Cx40 (green), e Cx43 (red)/Cx45 (green), and f AFP (red)/cTnT (green). Scale bar: 50 mum. c Expression of GATA4, a cardiac TF, was observed in both HOs ( n = 8 out of n = 9, two experiments) and E12.5 heart. d Expression of Cx40 in atrium-like region detected in the HOs ( n = 7 out of n = 13, two experiments) was similar to that of atrium in E13.5 heart. e The organized distribution of two types of Connexin (Cx43 and Cx45) in the HOs (over n = 8, two experiments) was similar to that of E12.5 heart (the ventricle part), although a small number of Cx45 (green)-positive cells was observed. f No expression of AFP (a liver marker) was detected in both HOs ( n = 8, over two biologically independent experiments) and E12.5 heart.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Molecular characterization of arteriole and post-arterial capillary endothelial cells. Exclusive expression of the arterial marker GJA5 by BVs (white arrowheads ( A )) and its overlay with HEY1 ( B ). The expression of ASS1 ( C ) and S100A4 ( D ) was restricted to the A and PAC clusters (white arrowheads). ( E ) Differential localization of ACKR1 and ASS1 discerns arterioles and post-arterial capillaries. Representative confocal images of section (upper) and whole-mount staining (lower) of ERG (green), ASS1 (red) and ACKR1 (white). Scale bars: 100 um.

37-8900

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