Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Blocking/Neutralizing [2]
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Validation data
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- Product number
- AF3209 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal BMP-9 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human BMP-9 in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant mouse BMP-9 is observed. In direct ELISAs and Western blots, less than 2% cross-reactivity with recombinant human (rh) BMP-2, -3, -4, -5, -7, -8, and rhBMP-10 is observed.
- Reactivity
- Human, Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Adenovirus‑mediated overexpression of bone morphogenetic protein‑9 promotes methionine choline deficiency‑induced non‑alcoholic steatohepatitis in non‑obese mice.
Li Q, Liu B, Breitkopf-Heinlein K, Weng H, Jiang Q, Dong P, Dooley S, Xu K, Ding H
Molecular medicine reports 2019 Sep;20(3):2743-2753
Molecular medicine reports 2019 Sep;20(3):2743-2753
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Alkaline Phosphatase Production Induced by BMP-9 and Neutralization by Human BMP-9 Antibody. Recombinant Human BMP-9 (Catalog # 3209-BP) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Alkaline phospha-tase production elicited by Recombinant Human BMP-9 (2 ng/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Human BMP-9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3209). The ND50 is typically 0.08-0.3 µg/mL.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Alkaline Phosphatase Production Induced by BMP‑9 and Neutralization by Human BMP‑9 Antibody. Alkaline Phosphatase Production Induced by BMP‑9 and Neutralization by Human BMP‑9 Antibody.