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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- PA1-28423 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Phospho-FAK (Ser910) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA1-28423 detects FAK from human samples. PA1-28423 has been successfully used in Western blot applications. The PA1-28423 immunogen is: Synthetic peptide derived from the region of FAK that contains serine 910. The sequence is conserved in human, mouse, rat, chicken and frog.
- Reactivity
- Human, Mouse, Rat, Xenopus
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot detection of Phospho-FAK pSer910 in extracts of human epithelial carcinoma cells expressing FAK with (lanes 2-5) or without (lane 1) 100 ng/mL Taxol for 18-24 hours in serum-free media to arrest cells in mitosis. Samples were resolved by SDS-PAGE on a 10% Tris-glycine gel, transferred to PVDF and incubated using a Phospho-FAK pSer910 polyclonal antibody (Product # PA1-28423).
