Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- GTX24800 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX24800, RRID:AB_380429
- Product name
- PYK2 (phospho Tyr402) antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat, Chicken/Avian
- Host
- Rabbit
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- Pyk2 [pY402] and [pY881] phosphospecific antibodies (GTX24801). Pyk2 [pY402] and [pY881] phosphospecific antibodies (GTX24801). Increased Pyk2 phosphorylation by SDF-1a treatment (5 min) of Reh cells, detected using Pyk2 [pY402] and Pyk2 [pY881] PSSAs. (GTX24801).
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Image shows auto-phosphorylation in TGF-beta treated NM?MG cells using Pyk2 [pY402] PSSA. (GTX24800)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of PYK2 [pY402] was done on 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton? X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with PYK2 [pY402] Rabbit Polyclonal Antibody at 1ug/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal? Secondary Antibody, Alexa Fluor? 488 conjugate at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade? Gold Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor? 555 Rhodamine Phalloidin. Panel d is a merged image showing cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Flow cytometry analysis of PYK2 [pY402] was done on PC-3 cells treated with Pervanadate (100uM, 1 hour). Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton? X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with PYK2 [pY402] Rabbit Polyclonal Antibody ( red histogram) or with rabbit isotype control (pink histogram) at 3-5 ug/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor? 488 Goat Anti-Rabbit Secondary Antibody at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune? Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.