Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
- Other assay [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 701094 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FAK Recombinant Rabbit Monoclonal Antibody (5H18L19)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 5H18L19
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Opposing effects of in vitro differentiated macrophages sub-type on epithelial wound healing.
Targeting CXCR4 and FAK reverses doxorubicin resistance and suppresses invasion in non-small cell lung carcinoma.
Gindele JA, Mang S, Pairet N, Christ I, Gantner F, Schymeinsky J, Lamb DJ
PloS one 2017;12(9):e0184386
PloS one 2017;12(9):e0184386
Targeting CXCR4 and FAK reverses doxorubicin resistance and suppresses invasion in non-small cell lung carcinoma.
Dragoj M, Milosevic Z, Bankovic J, Tanic N, Pesic M, Stankovic T
Cellular oncology (Dordrecht) 2017 Feb;40(1):47-62
Cellular oncology (Dordrecht) 2017 Feb;40(1):47-62
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FAK in whole cell extracts of Jurkat (lane 1) and K562 (lane 2) cell lines using a FAK recombinant rabbit monoclonal antibody (Product # 701094) at a dilution of 1 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of FAK1 was done on 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes; permeabilized with 0.25% Triton X-100 for 10 minutes followed by blocking with 5% BSA for 1 hour at room temperature. The cells were incubated with FAK1 Recombinant Rabbit Monoclonal Antibody (Product # 701094) at 2 µg-4 µg in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor® 488 Goat anti-Rabbit IgG Secondary Antibody (Product # A-11008) at a dilution of 1:400 for 30 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 594 Phalloidin (Product # A12381). Panel d is a merged image showing membrane and cytoplasmic localization of FAK1. Panel e shows no primary antibody control. The images were captured at 20X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of FAK1 was done on A549 cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Tritonª X-100 for 20 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were labeled with ABfinityª FAK1 Recombinant Rabbit Monoclonal Antibody (701094, red histogram) or with rabbit isotype control (pink histogram) at 2 µg-4 µg/million cells in 2.5% BSA. After incubation at room temperature for 2-3 hours, the cells were labeled with Alexa Fluor¨ 488 Goat Anti-Rabbit Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune¨ Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig 3 IHC of scratched cells and FN1 regulation/expression. A scratched epithelial cells incubated with different macrophage subtypes after 24h and 72h; DAPI stained in blue, FAK stained in yellow, FN1 stained in red, F-Actin stained in green. B FN1 mRNA regulation in the co-culture cell lysates measured after 24h and 72h Data are displayed as fold regulation compared to control medium containing macrophage maturation factors; Data is expressed as mean +- SD; n = 3 C FN1 expression measured in the supernatants via ELISA after 24h and 72h; Data is expressed as mean +- SD; n = 3 (**** = p>0.0001)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig 2 IHC of scratched cells and fibronectin expression. A IHC microscopic images of scratched cells at t = 0h, 6h, 24h, 48h and 72h; DAPI in blue, KRT5 in yellow, Ki67 in red. B IHC microscopic images of scratched cells at t = 0h, 6h, 24h, 48h and 72h; DAPI in blue, FAK in yellow, FN1 in red. C ELISA of FN1 in the supernatant after 0h, 6h, 24h, 48h and 72h with and without scratch.