Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-37706 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-FAK (Tyr576, Tyr577) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control for Western blot is HT29 cells; suggested positive control for ICC/IF is Hela cells.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Expression of p53 and its mechanism in prostate cancer.
Wan J, Zhang J, Zhang J
Oncology letters 2018 Jul;16(1):378-382
Oncology letters 2018 Jul;16(1):378-382
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts from HT29 cells, untreated or treated with PMA, using FAK (pTyr576/Tyr577) polyclonal antibody (Product # PA5-37706).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of Phospho-FAK (Tyr576, Tyr577) in methanol-fixed Hela cells, using Phospho-FAK (Tyr576, Tyr577) Polyclonal Antibody (Product # PA5-37706).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4. Western blotting was used to demonstrate that FAK-Src signaling is activated by si-p53. FAK, focal adhesion kinase; Src, Src proto-oncogene, non-receptor tyrosine kinase; si-p53, small interfering RNA targeting p53; p53, tumor protein p53; ERK, extracellular-regulated kinase; p-, phosphorylated; JNK, janus kinase; P-925, Phospho-FAK Tyr925; P-577, Phospho-FAK Tyr577; P-397, Phospho-FAK Tyr397.