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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Flow cytometry [1]
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Validation data
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- Product number
- 44-590G - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-FAK (Ser732) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Storage
- -20°C
Submitted references Identification of small molecules that interfere with radial neuronal migration and early cortical plate development.
Analyzing FAK and Pyk2 in early integrin signaling events.
Zhou L, Jossin Y, Goffinet AM
Cerebral cortex (New York, N.Y. : 1991) 2007 Jan;17(1):211-20
Cerebral cortex (New York, N.Y. : 1991) 2007 Jan;17(1):211-20
Analyzing FAK and Pyk2 in early integrin signaling events.
Bernard-Trifilo JA, Lim ST, Hou S, Schlaepfer DD, Ilic D
Current protocols in cell biology 2006 Apr;Chapter 14:Unit 14.7
Current protocols in cell biology 2006 Apr;Chapter 14:Unit 14.7
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of A549 (Lane 1) and A549 treated with VEGF (5 ng/mL for 5 minutes) (Lane 2). The blot was probed with Anti-Phospho-FAK (Ser732) Rabbit Polyclonal Antibody (Product# 44-590G, 1:500 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25µg/mL, 1:4000 dilution). A 121 kDa band corresponding to Phospho-FAK (Ser732) was observed upon VEGF treatment in A549 cell line.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of FAK [pS732] was done on A549 cells treated with EGF (200ng/mL, 10 minutes). Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with FAK [pS732] Rabbit Polyclonal Antibody (44590G, red histogram) or with rabbit isotype control (pink histogram) at 3-5 ug/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Goat Anti-Rabbit Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.
