Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
- Other assay [2]
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- Product number
- PA5-17084 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-FAK (Tyr397) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- It is not recommended to aliquot this antibody.
- Reactivity
- Human, Mouse, Rat, Hamster, Porcine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 13 µg/mL
- Storage
- -20°C
Submitted references Subendothelial stiffness alters endothelial cell traction force generation while exerting a minimal effect on the transcriptome.
Distinct PKA regulatory subunits mediate PGE(2) inhibition of TGFβ-1-stimulated collagen I translation and myofibroblast differentiation.
Focal adhesion kinase as a mechanotransducer during rapid brain growth of the chick embryo.
Green tea epigallocatechin gallate exhibits anticancer effect in human pancreatic carcinoma cells via the inhibition of both focal adhesion kinase and insulin-like growth factor-I receptor.
Bastounis EE, Yeh YT, Theriot JA
Scientific reports 2019 Dec 3;9(1):18209
Scientific reports 2019 Dec 3;9(1):18209
Distinct PKA regulatory subunits mediate PGE(2) inhibition of TGFβ-1-stimulated collagen I translation and myofibroblast differentiation.
Wettlaufer SH, Penke LR, Okunishi K, Peters-Golden M
American journal of physiology. Lung cellular and molecular physiology 2017 Oct 1;313(4):L722-L731
American journal of physiology. Lung cellular and molecular physiology 2017 Oct 1;313(4):L722-L731
Focal adhesion kinase as a mechanotransducer during rapid brain growth of the chick embryo.
Desmond ME, Knepper JE, DiBenedetto AJ, Malaugh E, Callejo S, Carretero R, Alonso MI, Gato A
The International journal of developmental biology 2014;58(1):35-43
The International journal of developmental biology 2014;58(1):35-43
Green tea epigallocatechin gallate exhibits anticancer effect in human pancreatic carcinoma cells via the inhibition of both focal adhesion kinase and insulin-like growth factor-I receptor.
Vu HA, Beppu Y, Chi HT, Sasaki K, Yamamoto H, Xinh PT, Tanii T, Hara Y, Watanabe T, Sato Y, Ohdomari I
Journal of biomedicine & biotechnology 2010;2010:290516
Journal of biomedicine & biotechnology 2010;2010:290516
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-FAK pTyr397 in extracts from NIH/3T3 cells, untransfected or transfected with activated Src, using Phospho-FAK pTyr397 polyclonal antibody (Product # PA5-17084) (upper) or a PTK2 polyclonal antibody (lower).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 Post-transcriptional changes on ECs in monolayers grown on soft versus stiff hydrogels. ( a ) Western blots from whole HUVEC or HMEC-1 lysates of cells previously residing on soft gels (3 kPa) or stiff gels (70 kPa). Representative cropped blots are displayed and full-length blots can be found in the supplementary material (Supplementary Fig. S2 ). Each row shows a different protein whose expression, phosphorylation or conformation was probed, namely: active form of integrin beta1, total integrin beta1, p397FAK, total FAK, p-Vav2, total Vav2, p-p70S6K, total p70S6K, p-ERK, total ERK and GAPDH (used as loading control). Experiments were performed N = 3 times. ( b ) Bar plots show relative expression of the proteins probed in panel a for HUVEC cells residing on soft (blue) or stiff (red) gels. All measurements were normalized to GAPDH expression for each condition, and expressed as fold-change relative to the median expression level on soft substrates. One or two asterisks denote statistically significant differences between the medians of two distributions (
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 4. PKA subunit RIIbeta agonist inhibits TGFbeta-1-stimulated phosphorylation of FAK and relative expression of SRF. A and B : reduced level of phosphorylation of FAK by PKARIIbeta agonist (Sp-5,6-DCI-cBIMPs) ( B ) but not PKARIbeta agonist (2-Cl-8-MA-cAMP) ( A ). After 24 h of serum starvation, cells were treated with PKAR specific agonists (500 muM) for 30 min before the addition of TGFbeta-1 (5 ng/ml), and cells were harvested 24 h thereafter for Western blot analysis. Blots are from one representative experiment, and graph shows mean and SE values from densitometric analysis of n = 4 independent experiments for p-FAK. *Significant difference from TGFbeta-1 alone, P < 0.001. C and D : inhibition of SRF mRNA ( C ) and protein ( D ) expression by PKARIIbeta agonist. After 48 h of serum starvation, cells were treated with PKAR specific agonists (500 muM) for 30 min before the addition of TGFbeta-1 (5 ng/ml), and cells were harvested 24 h thereafter for mRNA analysis by qPCR and protein analysis by Western blot. The graph shows mean and SE values from relative expression of n = 4 independent experiments. Densitometry of SRF protein blots was normalized to GAPDH. Relative expression of SRF was normalized to GAPDH. E : effect of R isoform-specific knockdown on PGE 2 inhibition of FAK protein phosphorylation by TGFbeta-1 After incubation for 4 days with R isoform-specific siRNA, HLFs were treated for 30 min with or without PGE 2 (500 nM) before addit