AM00059PU-N

antibody from Acris Antibodies GmbH

Targeting: PTK2 FADK, FAK, FAK1, PPP1R71

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Antibody Data

Product number

AM00059PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AM00059PU-N, RRID:AB_1001327

Product name

anti FAK1 / PTK2 pTyr397 (incl. pos. control)

Antibody type

Monoclonal

Antigen

Synthetic phosphopetide conjugated to KLH.Epitope: Phosphotyrosine 397

Reactivity

Human, Mouse, Rat, Canine

Host

Mouse

Isotype

IgG

Antibody clone number

2D11

Vial size

0.1 mg

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Detection of endogenous FAK: Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with mab AM00059PU-N (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec).Lane 1: HeLa Lane 2: HepG2 Lane 3: HEK293 Lane 4: SH-SY5Y Lane 5: MDCK Lane 6: PC12 Lane 7: CMT 93 Lane 8: Neuro 2ALane 9: 3T3